TY - JOUR
T1 - Glycoprotein B subtyping of cytomegalovirus (CMV) in the vitreous of patients with AIDS and CMV retinitis
AU - Chern, Kenneth C.
AU - Chandler, Diane B.
AU - Martin, Daniel F.
AU - Kuppermann, Baruch D.
AU - Wolitz, Richard A.
AU - Margolis, Todd P.
N1 - Funding Information:
Received 26 January 1998; revised 5 May 1998. Presented in part: Association for Research in Vision and Ophthalmology annual meeting, May 1997, Fort Lauderdale, Florida (abstract 3199). This study has been approved by the Committee on Human Research of the University of California, San Francisco. Financial support: Heed Ophthalmic Foundation (K.C.C.), Research to Prevent Blindness Lew Wasserman Merit Award (T.P.M.), the Ralph and Sophie Heintz Laboratory Fund (T.P.M.), NIH (EY-02162 to T.P.M.), and Universi-tywide AIDS Research Program (T.P.M.). Reprints or correspondence: Dr. Todd P. Margolis, Francis I. Proctor Foundation, Box 0944, UCSF Medical Center, San Francisco, CA 94143-0944 ([email protected]).
PY - 1998
Y1 - 1998
N2 - A 550-bp region of the cytomegalovirus (CMV) glycoprotein B (gB) gene was amplified by polymerase chain reaction (PCR) from 141 vitreous specimens of 120 patients with AIDS and CMV retinitis from three different metropolitan centers. The distribution of gB subtypes I, II, III, and IV were 19%, 43 %, 12%, and 21%, respectively, based on restriction enzyme digestion patterns of PCR-amplified DNA. Two patients had simultaneous infection with two different gB subtypes. The ratio of gB subtypes was similar among the three geographically distinct patient populations. Two of 14 patients with bilateral vitreous specimens had different viral subtypes in each eye. In addition, different gB subtypes were observed in 1 of 6 patients with serial specimens. The ratio of different gB subtypes in the vitreous of patients with CMV retinitis is similar to that previously reported in the peripheral blood of patients with advanced AIDS.
AB - A 550-bp region of the cytomegalovirus (CMV) glycoprotein B (gB) gene was amplified by polymerase chain reaction (PCR) from 141 vitreous specimens of 120 patients with AIDS and CMV retinitis from three different metropolitan centers. The distribution of gB subtypes I, II, III, and IV were 19%, 43 %, 12%, and 21%, respectively, based on restriction enzyme digestion patterns of PCR-amplified DNA. Two patients had simultaneous infection with two different gB subtypes. The ratio of gB subtypes was similar among the three geographically distinct patient populations. Two of 14 patients with bilateral vitreous specimens had different viral subtypes in each eye. In addition, different gB subtypes were observed in 1 of 6 patients with serial specimens. The ratio of different gB subtypes in the vitreous of patients with CMV retinitis is similar to that previously reported in the peripheral blood of patients with advanced AIDS.
UR - http://www.scopus.com/inward/record.url?scp=0031657999&partnerID=8YFLogxK
U2 - 10.1086/515672
DO - 10.1086/515672
M3 - Article
C2 - 9806048
AN - SCOPUS:0031657999
SN - 0022-1899
VL - 178
SP - 1149
EP - 1153
JO - Journal of Infectious Diseases
JF - Journal of Infectious Diseases
IS - 4
ER -