TY - JOUR
T1 - Glyco-engineering O-Antigen-Based Vaccines and Diagnostics in E. coli
AU - Vozza, Nicolas F.
AU - Feldman, Mario F.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - The biotechnological relevance of protein glycosylation has exponentially grown in recent years. With the advances in protein glycosylation research, new possibilities for glyco-engineering have arisen, and a wide array of glycans can be designed and potentially transferred to target proteins in the biotechnologically relevant host Escherichia coli. Here we provide insight on how to select the best strains and plasmids. We also describe methods for determination of glycan expression and assembly, protein glycosylation using western blot, and preparation of samples for mass spectrometry.
AB - The biotechnological relevance of protein glycosylation has exponentially grown in recent years. With the advances in protein glycosylation research, new possibilities for glyco-engineering have arisen, and a wide array of glycans can be designed and potentially transferred to target proteins in the biotechnologically relevant host Escherichia coli. Here we provide insight on how to select the best strains and plasmids. We also describe methods for determination of glycan expression and assembly, protein glycosylation using western blot, and preparation of samples for mass spectrometry.
UR - http://www.scopus.com/inward/record.url?scp=84957367070&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-2760-9_5
DO - 10.1007/978-1-4939-2760-9_5
M3 - Article
C2 - 26082215
AN - SCOPUS:84935843758
SN - 1064-3745
VL - 1321
SP - 57
EP - 70
JO - Methods in molecular biology (Clifton, N.J.)
JF - Methods in molecular biology (Clifton, N.J.)
ER -