TY - JOUR
T1 - Glycan array analysis of the antigen repertoire targeted by tumor-binding antibodies
AU - Gildersleeve, Jeffrey C.
AU - Wang, Baomei
AU - Achilefu, Samuel
AU - Tu, Zhude
AU - Xu, Mai
N1 - Funding Information:
We thank Jack Simpson (Protein Chemistry Laboratory, SAIC/NCI-Frederick) for MALDI-MS analysis of BSA conjugates. This research was supported in part by the Intramural Research Program of the NIH, NCI.
PY - 2012/11/15
Y1 - 2012/11/15
N2 - Immunization with whole cells has been used extensively to generate monoclonal antibodies, produce protective immune responses, and discover new disease antigens. While glycans are abundant on cell surfaces, anti-glycan immune responses have not been well-characterized. We used glycan microarrays to profile 49 tumor-binding monoclonal antibodies generated by immunizing mice with whole cancer cells. A substantial proportion (41%) of the tumor binding antibodies bound carbohydrate antigens. The antibodies primarily recognize a group of 5 glycan antigens: Sialyl Lewis A (SLeA), Lewis A (LeA), Lewis X (LeX), blood group A (BG-A), and blood group H on a type 2 chain (BG-H2). The results have important implications for monoclonal antibody production and cancer vaccine development.
AB - Immunization with whole cells has been used extensively to generate monoclonal antibodies, produce protective immune responses, and discover new disease antigens. While glycans are abundant on cell surfaces, anti-glycan immune responses have not been well-characterized. We used glycan microarrays to profile 49 tumor-binding monoclonal antibodies generated by immunizing mice with whole cancer cells. A substantial proportion (41%) of the tumor binding antibodies bound carbohydrate antigens. The antibodies primarily recognize a group of 5 glycan antigens: Sialyl Lewis A (SLeA), Lewis A (LeA), Lewis X (LeX), blood group A (BG-A), and blood group H on a type 2 chain (BG-H2). The results have important implications for monoclonal antibody production and cancer vaccine development.
KW - Antibody
KW - Cancer vaccine
KW - Carbohydrate
KW - Glycan array
KW - Tumor antigen
UR - http://www.scopus.com/inward/record.url?scp=84867889497&partnerID=8YFLogxK
U2 - 10.1016/j.bmcl.2012.09.055
DO - 10.1016/j.bmcl.2012.09.055
M3 - Article
C2 - 23063402
AN - SCOPUS:84867889497
SN - 0960-894X
VL - 22
SP - 6839
EP - 6843
JO - Bioorganic and Medicinal Chemistry Letters
JF - Bioorganic and Medicinal Chemistry Letters
IS - 22
ER -