Glycan array analysis of the antigen repertoire targeted by tumor-binding antibodies

Jeffrey C. Gildersleeve, Baomei Wang, Samuel Achilefu, Zhude Tu, Mai Xu

Research output: Contribution to journalArticle

15 Scopus citations

Abstract

Immunization with whole cells has been used extensively to generate monoclonal antibodies, produce protective immune responses, and discover new disease antigens. While glycans are abundant on cell surfaces, anti-glycan immune responses have not been well-characterized. We used glycan microarrays to profile 49 tumor-binding monoclonal antibodies generated by immunizing mice with whole cancer cells. A substantial proportion (41%) of the tumor binding antibodies bound carbohydrate antigens. The antibodies primarily recognize a group of 5 glycan antigens: Sialyl Lewis A (SLeA), Lewis A (LeA), Lewis X (LeX), blood group A (BG-A), and blood group H on a type 2 chain (BG-H2). The results have important implications for monoclonal antibody production and cancer vaccine development.

Original languageEnglish
Pages (from-to)6839-6843
Number of pages5
JournalBioorganic and Medicinal Chemistry Letters
Volume22
Issue number22
DOIs
StatePublished - Nov 15 2012

Keywords

  • Antibody
  • Cancer vaccine
  • Carbohydrate
  • Glycan array
  • Tumor antigen

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