TY - JOUR
T1 - Glutathione plays a role in the chick intestinal calcium absorption
AU - De Talamoni, Nori Tolosa
AU - Marchionatti, A.
AU - Baudino, V.
AU - Alisio, A.
PY - 1996/10
Y1 - 1996/10
N2 - DL-buthionine-S,R-sulfoximine (BSO) administration to vitamin D-deficient chicks treated with cholecalciferol produces a rapid decrease in the Ca2+ transfer from lumen-to-plasma and in the intestinal glutathione content. This response was reversed by addition of glutathione monoester to the intestinal sac. Variables related to the Ca2+ homeostasis such as plasma Ca and P, and intestinal calbindin D(28k) were not modified by BSO given to vitamin D-deficient chicks treated with cholecalciferol. Intestinal alkaline phosphatase activity, on the contrary, was highly reduced by BSO in vitamin D-deficient chicks treated with vitamin D3. This effect showed time and dose-dependency. Although the mechanism/s of action of BSO on the intestinal Ca absorption is unknown, it is quite possible that thiol groups of proteins involved in the Ca2+ transport are affected by the GSH depletion and/or by block of the antioxidant ability of vitamin D3. Thus, reactive oxygen compounds would be increased and, therefore, the Ca2+ movement from lumen to plasma decreases.
AB - DL-buthionine-S,R-sulfoximine (BSO) administration to vitamin D-deficient chicks treated with cholecalciferol produces a rapid decrease in the Ca2+ transfer from lumen-to-plasma and in the intestinal glutathione content. This response was reversed by addition of glutathione monoester to the intestinal sac. Variables related to the Ca2+ homeostasis such as plasma Ca and P, and intestinal calbindin D(28k) were not modified by BSO given to vitamin D-deficient chicks treated with cholecalciferol. Intestinal alkaline phosphatase activity, on the contrary, was highly reduced by BSO in vitamin D-deficient chicks treated with vitamin D3. This effect showed time and dose-dependency. Although the mechanism/s of action of BSO on the intestinal Ca absorption is unknown, it is quite possible that thiol groups of proteins involved in the Ca2+ transport are affected by the GSH depletion and/or by block of the antioxidant ability of vitamin D3. Thus, reactive oxygen compounds would be increased and, therefore, the Ca2+ movement from lumen to plasma decreases.
KW - alkaline phosphatase
KW - basolateral membrane
KW - Ca transfer from lumen-to-blood
KW - Ca uptake
KW - chicks
KW - cholecalciferol
KW - DL-buthionine-S,R-sulfoximine
KW - glutathion e
KW - intestine
UR - http://www.scopus.com/inward/record.url?scp=0030271191&partnerID=8YFLogxK
U2 - 10.1016/0300-9629(96)00023-0
DO - 10.1016/0300-9629(96)00023-0
M3 - Article
C2 - 8916550
AN - SCOPUS:0030271191
SN - 0300-9629
VL - 115
SP - 127
EP - 132
JO - Comparative Biochemistry and Physiology - A Physiology
JF - Comparative Biochemistry and Physiology - A Physiology
IS - 2
ER -