TY - JOUR
T1 - Glutamic acid decarboxylase mRNA in rat brain
T2 - regional distribution and effects of intrastriatal kainic acid
AU - Kim, Yong Sik
AU - Thomas, John W.
AU - Tillakaratne, Niranjala J.K.
AU - Montpied, Pascale
AU - Suzdak, Peter D.
AU - Banner, Carl
AU - Ginns, Edward
AU - Tobin, Allan J.
AU - Paul, Steven M.
N1 - Funding Information:
This work was in part supported by grants from the National Institutes of Health (NS22256 and NS20356)t o A.J.T.
PY - 1987/12
Y1 - 1987/12
N2 - Glutamic acid decarboxylase (GAD) mRNA was quantified in different regions of rat brain using an antisense RNA probe (riboprobe) prepared from a cloned feline cDNA. In all brain regions studied a single band of GAD mRNA of approximately 3.7 kb was detected. The level of GAD mRNA was highest in the cerebellum, followed by the hypothalamus > thalamus > striatum > hippocampus > frontal cortex = parietal cortex ≥ medulla = pons. Since GAD has been previously localized to intrinsic neurons of the striatum, we examined the effects of intrastriatal kainic acid administration on striatal GAD mRNA. The level of GAD mRNA in the kainic acid-lesioned striatum was reduced by 70-75% when compared to the contralateral (unlesioned) striatum. In contrast, the level of glutamine synthetase (an enzyme localized to glia) mRNA was increased approximately 290% in the kainic acid-lesioned striatum. There were no significant differences in GAD mRNA levels between the ipsilateral and contralateral cerebral cortices and hippocampi of rats injected with intrastriatal kainic acid.
AB - Glutamic acid decarboxylase (GAD) mRNA was quantified in different regions of rat brain using an antisense RNA probe (riboprobe) prepared from a cloned feline cDNA. In all brain regions studied a single band of GAD mRNA of approximately 3.7 kb was detected. The level of GAD mRNA was highest in the cerebellum, followed by the hypothalamus > thalamus > striatum > hippocampus > frontal cortex = parietal cortex ≥ medulla = pons. Since GAD has been previously localized to intrinsic neurons of the striatum, we examined the effects of intrastriatal kainic acid administration on striatal GAD mRNA. The level of GAD mRNA in the kainic acid-lesioned striatum was reduced by 70-75% when compared to the contralateral (unlesioned) striatum. In contrast, the level of glutamine synthetase (an enzyme localized to glia) mRNA was increased approximately 290% in the kainic acid-lesioned striatum. There were no significant differences in GAD mRNA levels between the ipsilateral and contralateral cerebral cortices and hippocampi of rats injected with intrastriatal kainic acid.
KW - Glutamic acid decarboxylase (GAD)
KW - Glutamine synthetase (GS)
KW - Kainic acid
KW - Messenger RNA (mRNA)
KW - Regional distribution
UR - http://www.scopus.com/inward/record.url?scp=0023567305&partnerID=8YFLogxK
U2 - 10.1016/0169-328X(87)90047-7
DO - 10.1016/0169-328X(87)90047-7
M3 - Article
C2 - 2448011
AN - SCOPUS:0023567305
SN - 0169-328X
VL - 3
SP - 77
EP - 82
JO - Molecular Brain Research
JF - Molecular Brain Research
IS - 1
ER -