GLUT-1 or GLUT-4 transgenes in obese mice improve glucose tolerance but do not prevent insulin resistance

Bess Adkins Marshall, Polly A. Hansen, Nancy J. Ensor, M. Allison Ogden, Mike Mueckler

Research output: Contribution to journalArticlepeer-review

28 Scopus citations

Abstract

Insulin-stimulated glucose uptake is defective in patients with type 2 diabetes. To determine whether transgenic glucose transporter overexpression in muscle can prevent diabetes induced by a high-fat, high-sugar diet, singly (GLUT-1, GLUT-4) and doubly (GLUT-1 and -4) transgenic mice were placed on a high-fat, high-sugar diet or a standard chow diet. On the high-fat, high- sugar diet, wild-type but not transgenic mice developed fasting hyperglycemia and glucose intolerance (peak glucose of 337 ± 19 vs. 185-209 mg/dl in the same groups on the high-fat, high-sugar diet and 293 ± 13 vs. 166-194 mg/dl on standard chow). Hyperinsulinemic clamps showed that transporter overexpression elevated insulin-stimulated glucose utilization on standard chow (49 ± 4 mg · kg-1 · min-1 in wild-type vs. 61 ± 4, 67 ± 5, and 63 ± 6 mg · kg-1 · min-1 in GLUT-l, GLUT-4, and GLUT-1 and -4 transgenic mice given 20 mU · kg-1 · min-1 insulin, and 54 ± 7, 85 ± 4, and 98 ± 11 in wild-type, GLUT-1, and GLUT-4 mice given 60-80 mU · kg- 1 · min-1 insulin). On the high-fat, high-sugar diet, wild-type and GLUT- 1 mice developed marked insulin resistance, but GLUT-4 and GLUT-1 and -4 mice were somewhat protected (glucose utilization during hyperinsulinemic clamp of 28.5 ± 3.4 vs. 42.4 ± 5.9, 51.2 ± 8.1, and 55.9 ± 4.9 mg · kg-1 · min-1 in wild type, GLUT-1, GLUT-4, GLUT-1 and -4 mice). These data demonstrate that overexpression of GLUT-1 and/or GLUT-4 enhances whole body glucose utilization and prevents the development of fasting hyperglycemia and glucose intolerance induced by a high-fat, high-sugar diet. GLUT-4 overexpression improves the insulin resistance induced by the diet. We conclude that upregulation of glucose transporters in skeletal muscle may be an effective therapeutic approach to the treatment of human type 2 diabetes.

Original languageEnglish
Pages (from-to)E390-E400
JournalAmerican Journal of Physiology - Endocrinology and Metabolism
Volume276
Issue number2 39-2
DOIs
StatePublished - Feb 1999

Keywords

  • Diabetes mellitus
  • Glucose clamp technique
  • Glucose transport
  • Obesity
  • Transgenic mice

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