TY - JOUR
T1 - Glucose-induced accumulation of inositol triphosphates in isolated pancreatic islets. Predominance of the 1,3,4-isomer
AU - Turk, J.
AU - Wolf, B. A.
AU - McDaniel, M. L.
PY - 1986
Y1 - 1986
N2 - Anion-exchange h.p.l.c. analysis of [3H]inositol phosphates derived from glucose-stimulated isolated pancreatic islets that had been prelabelled with myo-[3H]inositol revealed that the predominant inositol trisphosphate was the 1,3,4-isomer [Ins(1,3,4)P3]. The 1,4,5-isomer [Ins(1,4,5)P3] was also detectable, as was a more polar inositol phosphate with the chromatographic properties of inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4]. Glucose-induced accumulation of Ins(1,3,4)P3 was augmented by Li+ and occurred after maximal accumulation of Ins(1,4,5)P3. These findings suggest a possible role for Ins(1,3,4)P3 or its probable precursor Ins(1,3,4,5)P4 in stimulus-secretion coupling in pancreatic islets.
AB - Anion-exchange h.p.l.c. analysis of [3H]inositol phosphates derived from glucose-stimulated isolated pancreatic islets that had been prelabelled with myo-[3H]inositol revealed that the predominant inositol trisphosphate was the 1,3,4-isomer [Ins(1,3,4)P3]. The 1,4,5-isomer [Ins(1,4,5)P3] was also detectable, as was a more polar inositol phosphate with the chromatographic properties of inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4]. Glucose-induced accumulation of Ins(1,3,4)P3 was augmented by Li+ and occurred after maximal accumulation of Ins(1,4,5)P3. These findings suggest a possible role for Ins(1,3,4)P3 or its probable precursor Ins(1,3,4,5)P4 in stimulus-secretion coupling in pancreatic islets.
UR - http://www.scopus.com/inward/record.url?scp=0022541741&partnerID=8YFLogxK
U2 - 10.1042/bj2370259
DO - 10.1042/bj2370259
M3 - Article
C2 - 3541896
AN - SCOPUS:0022541741
SN - 0264-6021
VL - 237
SP - 259
EP - 263
JO - Biochemical Journal
JF - Biochemical Journal
IS - 1
ER -