Glucose-induced accumulation of inositol triphosphates in isolated pancreatic islets. Predominance of the 1,3,4-isomer

J. Turk, B. A. Wolf, M. L. McDaniel

Research output: Contribution to journalArticle

38 Scopus citations

Abstract

Anion-exchange h.p.l.c. analysis of [3H]inositol phosphates derived from glucose-stimulated isolated pancreatic islets that had been prelabelled with myo-[3H]inositol revealed that the predominant inositol trisphosphate was the 1,3,4-isomer [Ins(1,3,4)P3]. The 1,4,5-isomer [Ins(1,4,5)P3] was also detectable, as was a more polar inositol phosphate with the chromatographic properties of inositol 1,3,4,5-tetrakisphosphate [Ins(1,3,4,5)P4]. Glucose-induced accumulation of Ins(1,3,4)P3 was augmented by Li+ and occurred after maximal accumulation of Ins(1,4,5)P3. These findings suggest a possible role for Ins(1,3,4)P3 or its probable precursor Ins(1,3,4,5)P4 in stimulus-secretion coupling in pancreatic islets.

Original languageEnglish
Pages (from-to)259-263
Number of pages5
JournalBiochemical Journal
Volume237
Issue number1
DOIs
StatePublished - Jan 1 1986

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