Globin gene expression in somatic cell hybrids.

W. F. Anderson, Y. L. Chiang, L. Sanders-Haigh, T. J. Ley

Research output: Contribution to journalArticle

1 Scopus citations

Abstract

Fusions between somatic cell lines have previously yielded evidence for the existence of trans-acting gene regulatory factors. For this reason, we developed a cell line containing a "locked in" human 11-X translocation chromosome (containing the beta-globin-like gene cluster) in MEL cells. The human 11-X chromosome is stably integrated in the "M11-X" cell line, and single-copy human gamma and beta genes are present. After induction with HMBA, M11-X cells produced 500 copies per cell of correctly initiated, processed, and terminated human beta-globin mRNA; authentic human beta-globin chains were also produced at a low level. Despite the presence of normally arranged human gamma-globin genes, no gamma-globin mRNA could be detected after HMBA induction. However, cytosine residues near the gamma-globin gene promoters are completely methylated in these cells, suggesting that the gamma-globin genes may be repressed in part by DNA methylation. The pattern of human globin gene expression in M11-X cells may be affected by methylation and/or by trans-acting factors produced by these tetraploid cells.

Original languageEnglish
Pages (from-to)39-52
Number of pages14
JournalProgress in Clinical and Biological Research
Volume134
StatePublished - Dec 1 1983
Externally publishedYes

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    Anderson, W. F., Chiang, Y. L., Sanders-Haigh, L., & Ley, T. J. (1983). Globin gene expression in somatic cell hybrids. Progress in Clinical and Biological Research, 134, 39-52.