TY - JOUR
T1 - Globin gene expression in erythroid human fetal liver cells
AU - Ley, T. J.
AU - Maloney, K. A.
AU - Gordon, J. I.
AU - Schwartz, A. L.
PY - 1989
Y1 - 1989
N2 - We measured steady-state levels of the human globin mRNAs in liver samples from several mid-gestational fetuses. RNA from the ε, γ, β, ζ, θ, and α globin genes were present in fetal liver samples isolated from 10-25-wk embryos. The abundance of all human globin mRNAs declined in older fetuses, presumably because of a gradual reduction in the proportion of erythroid precursors in the liver as development procedes. The γ:β globin mRNA ratio in 10-18-wk fetal erythroblasts was 6-7:1, and in adult erythroid bone marrow the ratio was 0.02:1. In fetal liver samples, the relative abundance of ε transcripts was < 1% that of γ, and ζ transcripts < 5% that of α. Embryonic transcripts declined in abundance during late fetal development and were not detected in newborn liver or adult erythroid bone marrow. θ globin mRNA also represented a minor species (< 1% that of α) in fetal liver samples, but in contrast to the embryonic mRNAs, was most abundant in adult marrow samples obtained from patients with erythroid hyperplasia. These results support the hypothesis that globin protein levels are regulated by the relative amounts of each globin mRNA at various stages of erythropoietic development.
AB - We measured steady-state levels of the human globin mRNAs in liver samples from several mid-gestational fetuses. RNA from the ε, γ, β, ζ, θ, and α globin genes were present in fetal liver samples isolated from 10-25-wk embryos. The abundance of all human globin mRNAs declined in older fetuses, presumably because of a gradual reduction in the proportion of erythroid precursors in the liver as development procedes. The γ:β globin mRNA ratio in 10-18-wk fetal erythroblasts was 6-7:1, and in adult erythroid bone marrow the ratio was 0.02:1. In fetal liver samples, the relative abundance of ε transcripts was < 1% that of γ, and ζ transcripts < 5% that of α. Embryonic transcripts declined in abundance during late fetal development and were not detected in newborn liver or adult erythroid bone marrow. θ globin mRNA also represented a minor species (< 1% that of α) in fetal liver samples, but in contrast to the embryonic mRNAs, was most abundant in adult marrow samples obtained from patients with erythroid hyperplasia. These results support the hypothesis that globin protein levels are regulated by the relative amounts of each globin mRNA at various stages of erythropoietic development.
UR - http://www.scopus.com/inward/record.url?scp=0024590289&partnerID=8YFLogxK
U2 - 10.1172/JCI113944
DO - 10.1172/JCI113944
M3 - Article
C2 - 2921315
AN - SCOPUS:0024590289
SN - 0021-9738
VL - 83
SP - 1032
EP - 1038
JO - Journal of Clinical Investigation
JF - Journal of Clinical Investigation
IS - 3
ER -