Global gene deletion analysis exploring yeast filamentous growth

Owen Ryan, Rebecca S. Shapiro, Christoph F. Kurat, David Mayhew, Anastasia Baryshnikova, Brian Chin, Zhen Yuan Lin, Michael J. Cox, Frederick Vizeacoumar, Doris Cheung, Sondra Bahr, Kyle Tsui, Faiza Tebbji, Adnane Sellam, Fabian Istel, Tobias Schwarzmüller, Todd B. Reynolds, Karl Kuchler, David K. Gifford, Malcolm WhitewayGuri Giaever, Corey Nislow, Michael Costanzo, Anne Claude Gingras, Robi David Mitra, Brenda Andrews, Gerald R. Fink, Leah E. Cowen, Charles Boone

Research output: Contribution to journalArticlepeer-review

146 Scopus citations

Abstract

The dimorphic switch from a single-cell budding yeast to a filamentous form enables Saccharomyces cerevisiae to forage for nutrients and the opportunistic pathogen Candida albicans to invade human tissues and evade the immune system. We constructed a genome-wide set of targeted deletion alleles and introduced them into a filamentous S. cerevisiae strain, S1278b. We identified genes involved in morphologically distinct forms of filamentation: haploid invasive growth, biofilm formation, and diploid pseudohyphal growth. Unique genes appear to underlie each program, but we also found core genes with general roles in filamentous growth, including MFG1 (YDL233w), whose product binds two morphogenetic transcription factors, Flo8 and Mss11, and functions as a critical transcriptional regulator of filamentous growth in both S. cerevisiae and C. albicans.

Original languageEnglish
Pages (from-to)1352-1356
Number of pages5
JournalScience
Volume337
Issue number6100
DOIs
StatePublished - Sep 14 2012

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