Global gene deletion analysis exploring yeast filamentous growth

Owen Ryan; Rebecca S. Shapiro; Christoph F. Kurat; David Mayhew; Anastasia Baryshnikova; Brian Chin; Zhen Yuan Lin; Michael J. Cox; Frederick Vizeacoumar; Doris Cheung; Sondra Bahr; Kyle Tsui; Faiza Tebbji; Adnane Sellam; Fabian Istel; Tobias Schwarzmüller; Todd B. Reynolds; Karl Kuchler; David K. Gifford; Malcolm Whiteway; Guri Giaever; Corey Nislow; Michael Costanzo; Anne Claude Gingras; Robi David Mitra; Brenda Andrews; Gerald R. Fink; Leah E. Cowen; Charles Boone

doi:10.1126/science.1224339

Global gene deletion analysis exploring yeast filamentous growth

Owen Ryan, Rebecca S. Shapiro, Christoph F. Kurat, David Mayhew, Anastasia Baryshnikova, Brian Chin, Zhen Yuan Lin, Michael J. Cox, Frederick Vizeacoumar, Doris Cheung, Sondra Bahr, Kyle Tsui, Faiza Tebbji, Adnane Sellam, Fabian Istel, Tobias Schwarzmüller, Todd B. Reynolds, Karl Kuchler, David K. Gifford, Malcolm WhitewayGuri Giaever, Corey Nislow, Michael Costanzo, Anne Claude Gingras, Robi David Mitra, Brenda Andrews, Gerald R. Fink, Leah E. Cowen, Charles Boone

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170 Scopus citations

Abstract

The dimorphic switch from a single-cell budding yeast to a filamentous form enables Saccharomyces cerevisiae to forage for nutrients and the opportunistic pathogen Candida albicans to invade human tissues and evade the immune system. We constructed a genome-wide set of targeted deletion alleles and introduced them into a filamentous S. cerevisiae strain, S1278b. We identified genes involved in morphologically distinct forms of filamentation: haploid invasive growth, biofilm formation, and diploid pseudohyphal growth. Unique genes appear to underlie each program, but we also found core genes with general roles in filamentous growth, including MFG1 (YDL233w), whose product binds two morphogenetic transcription factors, Flo8 and Mss11, and functions as a critical transcriptional regulator of filamentous growth in both S. cerevisiae and C. albicans.

Original language	English
Pages (from-to)	1352-1356
Number of pages	5
Journal	Science
Volume	337
Issue number	6100
DOIs	https://doi.org/10.1126/science.1224339
State	Published - Sep 14 2012

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Ryan, O., Shapiro, R. S., Kurat, C. F., Mayhew, D., Baryshnikova, A., Chin, B., Lin, Z. Y., Cox, M. J., Vizeacoumar, F., Cheung, D., Bahr, S., Tsui, K., Tebbji, F., Sellam, A., Istel, F., Schwarzmüller, T., Reynolds, T. B., Kuchler, K., Gifford, D. K., ... Boone, C. (2012). Global gene deletion analysis exploring yeast filamentous growth. Science, 337(6100), 1352-1356. https://doi.org/10.1126/science.1224339

@article{7d7af651525d404c8039045fe5cae322,

title = "Global gene deletion analysis exploring yeast filamentous growth",

abstract = "The dimorphic switch from a single-cell budding yeast to a filamentous form enables Saccharomyces cerevisiae to forage for nutrients and the opportunistic pathogen Candida albicans to invade human tissues and evade the immune system. We constructed a genome-wide set of targeted deletion alleles and introduced them into a filamentous S. cerevisiae strain, S1278b. We identified genes involved in morphologically distinct forms of filamentation: haploid invasive growth, biofilm formation, and diploid pseudohyphal growth. Unique genes appear to underlie each program, but we also found core genes with general roles in filamentous growth, including MFG1 (YDL233w), whose product binds two morphogenetic transcription factors, Flo8 and Mss11, and functions as a critical transcriptional regulator of filamentous growth in both S. cerevisiae and C. albicans.",

author = "Owen Ryan and Shapiro, {Rebecca S.} and Kurat, {Christoph F.} and David Mayhew and Anastasia Baryshnikova and Brian Chin and Lin, {Zhen Yuan} and Cox, {Michael J.} and Frederick Vizeacoumar and Doris Cheung and Sondra Bahr and Kyle Tsui and Faiza Tebbji and Adnane Sellam and Fabian Istel and Tobias Schwarzm{\"u}ller and Reynolds, {Todd B.} and Karl Kuchler and Gifford, {David K.} and Malcolm Whiteway and Guri Giaever and Corey Nislow and Michael Costanzo and Gingras, {Anne Claude} and Mitra, {Robi David} and Brenda Andrews and Fink, {Gerald R.} and Cowen, {Leah E.} and Charles Boone",

year = "2012",

month = sep,

day = "14",

doi = "10.1126/science.1224339",

language = "English",

volume = "337",

pages = "1352--1356",

journal = "Science",

issn = "0036-8075",

number = "6100",

}

Ryan, O, Shapiro, RS, Kurat, CF, Mayhew, D, Baryshnikova, A, Chin, B, Lin, ZY, Cox, MJ, Vizeacoumar, F, Cheung, D, Bahr, S, Tsui, K, Tebbji, F, Sellam, A, Istel, F, Schwarzmüller, T, Reynolds, TB, Kuchler, K, Gifford, DK, Whiteway, M, Giaever, G, Nislow, C, Costanzo, M, Gingras, AC, Mitra, RD, Andrews, B, Fink, GR, Cowen, LE & Boone, C 2012, 'Global gene deletion analysis exploring yeast filamentous growth', Science, vol. 337, no. 6100, pp. 1352-1356. https://doi.org/10.1126/science.1224339

TY - JOUR

T1 - Global gene deletion analysis exploring yeast filamentous growth

AU - Ryan, Owen

AU - Shapiro, Rebecca S.

AU - Kurat, Christoph F.

AU - Mayhew, David

AU - Baryshnikova, Anastasia

AU - Chin, Brian

AU - Lin, Zhen Yuan

AU - Cox, Michael J.

AU - Vizeacoumar, Frederick

AU - Cheung, Doris

AU - Bahr, Sondra

AU - Tsui, Kyle

AU - Tebbji, Faiza

AU - Sellam, Adnane

AU - Istel, Fabian

AU - Schwarzmüller, Tobias

AU - Reynolds, Todd B.

AU - Kuchler, Karl

AU - Gifford, David K.

AU - Whiteway, Malcolm

AU - Giaever, Guri

AU - Nislow, Corey

AU - Costanzo, Michael

AU - Gingras, Anne Claude

AU - Mitra, Robi David

AU - Andrews, Brenda

AU - Fink, Gerald R.

AU - Cowen, Leah E.

AU - Boone, Charles

PY - 2012/9/14

Y1 - 2012/9/14

N2 - The dimorphic switch from a single-cell budding yeast to a filamentous form enables Saccharomyces cerevisiae to forage for nutrients and the opportunistic pathogen Candida albicans to invade human tissues and evade the immune system. We constructed a genome-wide set of targeted deletion alleles and introduced them into a filamentous S. cerevisiae strain, S1278b. We identified genes involved in morphologically distinct forms of filamentation: haploid invasive growth, biofilm formation, and diploid pseudohyphal growth. Unique genes appear to underlie each program, but we also found core genes with general roles in filamentous growth, including MFG1 (YDL233w), whose product binds two morphogenetic transcription factors, Flo8 and Mss11, and functions as a critical transcriptional regulator of filamentous growth in both S. cerevisiae and C. albicans.

AB - The dimorphic switch from a single-cell budding yeast to a filamentous form enables Saccharomyces cerevisiae to forage for nutrients and the opportunistic pathogen Candida albicans to invade human tissues and evade the immune system. We constructed a genome-wide set of targeted deletion alleles and introduced them into a filamentous S. cerevisiae strain, S1278b. We identified genes involved in morphologically distinct forms of filamentation: haploid invasive growth, biofilm formation, and diploid pseudohyphal growth. Unique genes appear to underlie each program, but we also found core genes with general roles in filamentous growth, including MFG1 (YDL233w), whose product binds two morphogenetic transcription factors, Flo8 and Mss11, and functions as a critical transcriptional regulator of filamentous growth in both S. cerevisiae and C. albicans.

UR - http://www.scopus.com/inward/record.url?scp=84866124383&partnerID=8YFLogxK

U2 - 10.1126/science.1224339

DO - 10.1126/science.1224339

M3 - Article

C2 - 22984072

AN - SCOPUS:84866124383

SN - 0036-8075

VL - 337

SP - 1352

EP - 1356

JO - Science

JF - Science

IS - 6100

ER -

Global gene deletion analysis exploring yeast filamentous growth

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