TY - JOUR
T1 - Germline organization of the murine T cell receptor β-chain genes
AU - Chou, Hubert S.
AU - Nelson, Christopher A.
AU - Godambe, Sandip A.
AU - Chaplin, David D.
AU - Loh, Dennis Y.
PY - 1987
Y1 - 1987
N2 - The complete germline organization of the β-chain genes of the murine T cell receptor was elucidated in order to obtain the structural basis for understanding the mechanisms of somatic DNA rearrangements. Twenty of the 22 known variable (Vβ) genes are clustered within 250 kilobases of DNA 5′ to the constant region (Cβ) genes. These V β genes share the same transcriptional orientation as the diversity (Dβ), joining (Jβ), and C β genes, which implies that chromosomal deletion is the mechanism for most Vβ to Dβ-Jβ rearrangements. Within this Vβ cluster, the distance between the most proximal Vβ gene and the Dβ-J β-Cβ cluster is 320 kilobases, as determined by field-inversion gel electrophoresis. The large distance between V β and Dβ, relative to that between D β and Jβ, may have significant implications for the ordered rearrangement of the T cell receptor β-chain genes.
AB - The complete germline organization of the β-chain genes of the murine T cell receptor was elucidated in order to obtain the structural basis for understanding the mechanisms of somatic DNA rearrangements. Twenty of the 22 known variable (Vβ) genes are clustered within 250 kilobases of DNA 5′ to the constant region (Cβ) genes. These V β genes share the same transcriptional orientation as the diversity (Dβ), joining (Jβ), and C β genes, which implies that chromosomal deletion is the mechanism for most Vβ to Dβ-Jβ rearrangements. Within this Vβ cluster, the distance between the most proximal Vβ gene and the Dβ-J β-Cβ cluster is 320 kilobases, as determined by field-inversion gel electrophoresis. The large distance between V β and Dβ, relative to that between D β and Jβ, may have significant implications for the ordered rearrangement of the T cell receptor β-chain genes.
UR - http://www.scopus.com/inward/record.url?scp=0023640249&partnerID=8YFLogxK
U2 - 10.1126/science.2821625
DO - 10.1126/science.2821625
M3 - Article
C2 - 2821625
AN - SCOPUS:0023640249
SN - 0036-8075
VL - 238
SP - 545
EP - 548
JO - Science
JF - Science
IS - 4826
ER -