Germline ETV6 mutation promotes inflammation and disrupts lymphoid development of early hematopoietic progenitors

Chengjing Zhou, Rizvan Uluisik, Jesse W. Rowley, Camille David, Courtney L. Jones, Christopher D. Scharer, Leila Noetzli, Marlie H. Fisher, Gregory D. Kirkpatrick, Katrina Bark, Jeremy M. Boss, Curtis J. Henry, Eric M. Pietras, Jorge Di Paola, Christopher C. Porter

Research output: Contribution to journalArticlepeer-review

1 Scopus citations

Abstract

Germline mutations in ETV6 are associated with a syndrome of thrombocytopenia and leukemia predisposition, and ETV6 is among the most commonly mutated genes in leukemias, especially childhood B-cell acute lymphoblastic leukemia. However, the mechanisms underlying disease caused by ETV6 dysfunction are poorly understood. To address these gaps in knowledge, using CRISPR/Cas9, we developed a mouse model of the most common recurrent, disease-causing germline mutation in ETV6. We found defects in hematopoiesis related primarily to abnormalities of the multipotent progenitor population 4 (MPP4) subset of hematopoietic progenitor cells and evidence of sterile inflammation. Expression of ETV6 in Ba/F3 cells altered the expression of several cytokines, some of which were also detected at higher levels in the bone marrow of the mice with Etv6 mutation. Among these, interleukin-18 and interleukin-13 abrogated B-cell development of sorted MPP4 cells, but not common lymphoid progenitors, suggesting that inflammation contributes to abnormal hematopoiesis by impairing lymphoid development. These data, along with those from humans, support a model in which ETV6 dysfunction promotes inflammation, which adversely affects thrombopoiesis and promotes leukemogenesis.

Original languageEnglish
Pages (from-to)24-34
Number of pages11
JournalExperimental Hematology
Volume112-113
DOIs
StatePublished - Aug 1 2022

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