TY - JOUR
T1 - Gerbilline cholesteatoma development. Part II
T2 - Temporal histopathologic changes in the tympanic membrane and middle ear
AU - Tinling, Steven P.
AU - Chole, Richard A.
N1 - Funding Information:
Supported in part by grants from the Deafness Research Foundation and NIH Grant R01-NS21079.
PY - 2006/6
Y1 - 2006/6
N2 - Objective: Determine the sequence of gross and histopathologic change to the normal middle ear (ME), tympanic membrane (TM), and external auditory canal (EAC) during spontaneous gerbilline cholesteatoma development. Study design and setting: Sixty-six gerbils were examined weekly and periodically sacrificed for analysis. Results: Cholesteatoma development followed this sequence: 1) slightly thickened pars flaccida (PF) without ME effusion, 2) thickened PF with ME effusion, 3) continuous buildup of EAC debris, and 4) complete occlusion of the lateral EAC. In the cholesteatoma group, keratinizing epithelium (KE) thickness was increased in all regions over normal controls and regional gradations in the TM remained the same. Conclusion: Spontaneous cholesteatoma formation was significantly associated with middle ear effusion, a failure to clear canal debris with resultant retrograde thickening of the EAC, PF, and then pars tensa. Significance: Inflammatory insult and alteration of the epithelial clearance mechanism appears to result in spontaneous cholesteatoma formation in gerbils as hypothesized for humans.
AB - Objective: Determine the sequence of gross and histopathologic change to the normal middle ear (ME), tympanic membrane (TM), and external auditory canal (EAC) during spontaneous gerbilline cholesteatoma development. Study design and setting: Sixty-six gerbils were examined weekly and periodically sacrificed for analysis. Results: Cholesteatoma development followed this sequence: 1) slightly thickened pars flaccida (PF) without ME effusion, 2) thickened PF with ME effusion, 3) continuous buildup of EAC debris, and 4) complete occlusion of the lateral EAC. In the cholesteatoma group, keratinizing epithelium (KE) thickness was increased in all regions over normal controls and regional gradations in the TM remained the same. Conclusion: Spontaneous cholesteatoma formation was significantly associated with middle ear effusion, a failure to clear canal debris with resultant retrograde thickening of the EAC, PF, and then pars tensa. Significance: Inflammatory insult and alteration of the epithelial clearance mechanism appears to result in spontaneous cholesteatoma formation in gerbils as hypothesized for humans.
UR - http://www.scopus.com/inward/record.url?scp=33646786100&partnerID=8YFLogxK
U2 - 10.1016/j.otohns.2005.12.024
DO - 10.1016/j.otohns.2005.12.024
M3 - Article
C2 - 16730537
AN - SCOPUS:33646786100
SN - 0194-5998
VL - 134
SP - 953
EP - 960
JO - Otolaryngology - Head and Neck Surgery
JF - Otolaryngology - Head and Neck Surgery
IS - 6
ER -