TY - JOUR
T1 - Genomic structure of the EWS gene and its relationship to EWSR1, a site of tumor-associated chromosome translocation
AU - Plougastel, Béatrice
AU - Zucman, Jessica
AU - Peter, Martine
AU - Thomas, Gilles
AU - Delattre, Olivier
N1 - Funding Information:
This work was supported by grants from the European Community Commissions, the Ministere de la Recherche et de la Technologie, the Ligue Nationale contre le cancer, and the Council for Coordinating Cancer Research. B.P. and J.Z. are recipients of fellowships from the Ministere de la Recherche et de la Technologie and from the Fonda-tion pour la Recherche Medicale, respectively.
PY - 1993/12
Y1 - 1993/12
N2 - The EWS gene has been identified based on its locationat the chromosome 22 breakpoint of the t(11;22)(q24;q12) translocation that characterizes Ewing sarcoma and related neuroectodermal tumors. The EWS gene spans about 40 kb of DNA and is encoded by 17 exons. The nucleotide sequence of the exons is identical to that of the previously described cDNA. The first 7 exons encode the N-terminal domain of EWS, which consists of a repeated degenerated polypeptide of 7 to 12 residues rich in tyrosine, serine, threonine, glycine, and glutamine. Exons 11, 12, and 13 encode the putative RNA binding domain. The three glycine- and arginine-rich motifs of the gene are mainly encoded by exons 8-9, 14, and 16. The DNA sequence in the 5′ region of the gene has features of a CpG-rich island and lacks canonical promoter elements, such as TATA and CCAAT consensus sequences. Positions of the chromosome 22 breakpoints were determined for 19 Ewing tumors. They were localized in introns 7 or 8 in 18 cases and in intron 10 in 1 case.
AB - The EWS gene has been identified based on its locationat the chromosome 22 breakpoint of the t(11;22)(q24;q12) translocation that characterizes Ewing sarcoma and related neuroectodermal tumors. The EWS gene spans about 40 kb of DNA and is encoded by 17 exons. The nucleotide sequence of the exons is identical to that of the previously described cDNA. The first 7 exons encode the N-terminal domain of EWS, which consists of a repeated degenerated polypeptide of 7 to 12 residues rich in tyrosine, serine, threonine, glycine, and glutamine. Exons 11, 12, and 13 encode the putative RNA binding domain. The three glycine- and arginine-rich motifs of the gene are mainly encoded by exons 8-9, 14, and 16. The DNA sequence in the 5′ region of the gene has features of a CpG-rich island and lacks canonical promoter elements, such as TATA and CCAAT consensus sequences. Positions of the chromosome 22 breakpoints were determined for 19 Ewing tumors. They were localized in introns 7 or 8 in 18 cases and in intron 10 in 1 case.
UR - http://www.scopus.com/inward/record.url?scp=0027760934&partnerID=8YFLogxK
U2 - 10.1016/S0888-7543(05)80363-5
DO - 10.1016/S0888-7543(05)80363-5
M3 - Article
C2 - 8307570
AN - SCOPUS:0027760934
SN - 0888-7543
VL - 18
SP - 609
EP - 615
JO - Genomics
JF - Genomics
IS - 3
ER -