Genomic organization of mouse Fc(γ) receptor genes

A. Kulczycki, J. Webber, H. A. Soares, M. D. Onken, J. A. Thompson, D. D. Chaplin, D. Y. Loh, J. P. Tillinghast

Research output: Contribution to journalArticlepeer-review

21 Scopus citations

Abstract

We have isolated and characterized the gene coding for the mouse Fc receptor that is termed Fc(γ)RIIa. The gene contains five exons and spans approximately 9 kilobases. Unlike most members of the immunoglobulin gene superfamily, this gene utilizes multiple exons to encode its leader peptide. The first exon encodes the hydrophobic region of the signal sequence; the second exon, which contains only 21 base pairs, encodes a segment of the signal peptidase recognition site; and the beginning of the third exon encodes the predicted site of peptidase cleavage. The third and fourth exons each code for immunoglobulin-like extracellular domains. The fifth exon encodes the hydrophobic transmembrane domain and the cytoplasmic tail. Partial characterization of the Fc(γ)RIIb gene indicates that it also contains multiple leader exons, including a 21-base-pair exon and two exons coding for homologous immunoglobulin-like extracellular domains. However, the Fc(γ)RIIb gene uses four exons to encode its intracytoplasmic region. Analysis using contour-clamped homogeneous electric field (CHEF) gels indicates that the Fc(γ)RIIa and Fc(γ)RIIb genes are linked within 160 kilobases on mouse chromosome 1.

Original languageEnglish
Pages (from-to)2856-2860
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume87
Issue number7
DOIs
StatePublished - 1990

Keywords

  • Fc receptors
  • exon-intron junctions
  • immunoglobulin gene superfamily
  • protein domains

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