TY - JOUR
T1 - Genomic organization, nucleotide sequence, and cellular distribution of a Shaw-related potassium channel gene, Kv3.3, and mapping of Kv3.3 and Kv3.4 to human chromosomes 19 and 1
AU - Ghanshani, Sanjiv
AU - Pak, Michael
AU - McPherson, John D.
AU - Strong, Michael
AU - Dethlefs, Brent
AU - Wasmuth, John J.
AU - Salkoff, Lawrence
AU - Gutman, George A.
AU - Chandy, K. George
N1 - Funding Information:
(KCNC4) K+ channel genes to human chromosomes 19 and 1, The probe used for chromosomal localization is designated by the for chromosomal mapping studies is designated by the arrows. (C) Kv3.3 (KCNC3) and human Kv3.4 (KCNC4). The chromosome (65°C and 0.1X SSC) with the Shaw-related potassium channel UCI) for providing us with RNA. The nucleotide sequences reported herein have been submitted to GenBank and have been assigned accession numbers X60796 and X60797 for the two Kv3.3 exons and M81253 for Kv3.4. This work was supported by USPHS Grants AI24783 (K.G.C.), A1213166 (G.A.G.), and NF24785 (L.S.); Grant 188209 from the Juvenile Diabetes Foundation International (G.A.G.); a Muscular Dystrophy grant (L.S.); a grant from Pfizer, Inc., Groton, CT (K.G.C.); an NIH training grant (M.P.); and Grant HG00320 from the National Center for Human Genome Research (J.W.).
PY - 1992/2
Y1 - 1992/2
N2 - Genomic and cDNA clones encoding a novel Shaw-related potassium channel gene have been isolated from mice and humans. The mouse-Kv3.3 gene encodes a protein of 679 amino acids. Unlike the vertebrate Shaker-related genes that have intronless coding regions, mouse Kv3.3 is encoded by at least two exons separated by 3 kb of intervening sequence. The amino-terminal 212 amino acids are encoded by a single exon, and the hydrophobic core of the protein beginning at the S1 transmembrane segment is contained in a separate exon. Multiple Kv3.3-hybridizing transcripts are visible in the mouse brain, liver, thymus, and heart. Using probes derived from a human genomic clone containing the 3′ exon of human Kv3.3 (KCNC3), we have localized the gene to human chromosome 19. The related gene, human Kv3.4 (KCNC4), was localized to human chromosome 1.
AB - Genomic and cDNA clones encoding a novel Shaw-related potassium channel gene have been isolated from mice and humans. The mouse-Kv3.3 gene encodes a protein of 679 amino acids. Unlike the vertebrate Shaker-related genes that have intronless coding regions, mouse Kv3.3 is encoded by at least two exons separated by 3 kb of intervening sequence. The amino-terminal 212 amino acids are encoded by a single exon, and the hydrophobic core of the protein beginning at the S1 transmembrane segment is contained in a separate exon. Multiple Kv3.3-hybridizing transcripts are visible in the mouse brain, liver, thymus, and heart. Using probes derived from a human genomic clone containing the 3′ exon of human Kv3.3 (KCNC3), we have localized the gene to human chromosome 19. The related gene, human Kv3.4 (KCNC4), was localized to human chromosome 1.
UR - http://www.scopus.com/inward/record.url?scp=0026561880&partnerID=8YFLogxK
U2 - 10.1016/0888-7543(92)90365-Y
DO - 10.1016/0888-7543(92)90365-Y
M3 - Article
C2 - 1740329
AN - SCOPUS:0026561880
SN - 0888-7543
VL - 12
SP - 190
EP - 196
JO - Genomics
JF - Genomics
IS - 2
ER -