Genome-wide RNAi screen reveals a specific sensitivity of IRES-containing RNA viruses to host translation inhibition

Sara Cherry, Tammy Doukas, Susan Armknecht, Sean Whelan, Hui Wang, Peter Sarnow, Norbert Perrimon

Research output: Contribution to journalArticlepeer-review

184 Scopus citations

Abstract

The widespread class of RNA viruses that utilize internal ribosome entry sites (IRESs) for translation include poliovirus and Hepatitis C virus. To identify host factors required for IRES-dependent translation and viral replication, we performed a genome-wide RNAi screen in Drosophila cells infected with Drosophila C virus (DCV). We identified 66 ribosomal proteins that, when depleted, specifically inhibit DCV growth, but not a non-IRES-containing RNA virus. Moreover, treatment of flies with a translation inhibitor is protective in vivo. Finally, this increased sensitivity to ribosome levels also holds true for poliovirus infection of human cells, demonstrating the generality of these findings.

Original languageEnglish
Pages (from-to)445-452
Number of pages8
JournalGenes and Development
Volume19
Issue number4
DOIs
StatePublished - Feb 15 2005

Keywords

  • Dicistroviridae
  • Drosophila
  • Drosophila C virus
  • Picornavirus
  • Ribosome
  • Translation

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