Genome-wide RNAi screen for host factors required for intracellular bacterial infection

Hervé Agaisse, Laura S. Burrack, Jennifer A. Philips, Eric J. Rubin, Norbert Perrimon, Darren E. Higgins

Research output: Contribution to journalArticle

237 Scopus citations

Abstract

Most studies of host-pathogen interactions have focused on pathogen-specific virulence determinants. Here, we report a genome-wide RNA interference screen to identify host factors required for intracellular bacterial pathogenesis. Using Drosophila cells and the cytosolic pathogen Listeria monocytogenes, we identified 305 double-stranded RNAs targeting a wide range of cellular functions that altered L. monocytogenes infection. Comparison to a similar screen with Mycobacterium fortuitum, a vacuolar pathogen, identified host factors that may play a general role in intracellular pathogenesis and factors that specifically affect access to the cytosol by L. monocytogenes.

Original languageEnglish
Pages (from-to)1248-1251
Number of pages4
JournalScience
Volume309
Issue number5738
DOIs
StatePublished - Aug 19 2005
Externally publishedYes

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    Agaisse, H., Burrack, L. S., Philips, J. A., Rubin, E. J., Perrimon, N., & Higgins, D. E. (2005). Genome-wide RNAi screen for host factors required for intracellular bacterial infection. Science, 309(5738), 1248-1251. https://doi.org/10.1126/science.1116008