Genome-wide identification of NBS resistance genes in Populus trichocarpa

Annegret Kohler, Cécile Rinaldi, Sébastien Duplessis, Marie Baucher, Danny Geelen, Frédéric Duchaussoy, Blake C. Meyers, Wout Boerjan, Francis Martin

Research output: Contribution to journalArticlepeer-review

202 Scopus citations

Abstract

As the largest class of disease resistance R genes, the genes encoding nucleotide binding site and leucine-rich repeat proteins ("NBS-LRR genes") play a critical role in defending plants from a multitude of pathogens and pests. The diversity of NBS-LRR genes was examined in the Populus trichocarpa draft genome sequence. The NBS class of genes in this perennial tree is large and diverse, comprised of ∼400 genes, at least twice the complement of Arabidopsis. The NBS family can be divided into multiple subfamilies with distinct domain organizations. It includes 119 Coiled-Coil-NBS-LRR genes, 64 TIR-NBS-LRR genes, 34 BED-finger-NBS-LRR, and both truncated and unusual NBS- and NBS-LRR-containing genes. The transcripts of only 34 NBS-LRR genes were detected in rust-infected and non-infected leaves using a whole-genome oligoarray. None showed an altered expression two days post inoculation.

Original languageEnglish
Pages (from-to)619-636
Number of pages18
JournalPlant Molecular Biology
Volume66
Issue number6
DOIs
StatePublished - Apr 2008

Keywords

  • NBS-LRR
  • Populus
  • Resistance

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