Genome-wide analysis of electrical phenotype using isodielectric separation

M. D. Vahey, J. P. Svensson, L. Quiros Pesudo, L. D. Samson, J. Voldman

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

We present the first genome-wide analysis of electrical phenotype, focusing on the budding yeast Saccharomyces ce-revisiae. Using isodielectric separation (IDS), a microfluidic platform recently developed by our lab, we have screened the haploid yeast knock-out collection to identify genes whose deletion confers an increase or decrease in effective conductivity relative to wildtype cells. Pathway analysis and subsequent characterization of these deletion strains suggest biological processes and genetic pathways associated with distinct electrical phenotype. These results demonstrate the feasibility of IDS as a platform for genetic screens and establish a new class of phenotypic analysis - electrogenomic profiling.

Original languageEnglish
Title of host publication14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Pages725-727
Number of pages3
StatePublished - 2010
Event14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010 - Groningen, Netherlands
Duration: Oct 3 2010Oct 7 2010

Publication series

Name14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Volume2

Conference

Conference14th International Conference on Miniaturized Systems for Chemistry and Life Sciences 2010, MicroTAS 2010
Country/TerritoryNetherlands
CityGroningen
Period10/3/1010/7/10

Keywords

  • Cell Separation
  • Dielectrophoresis
  • Electrical Properties
  • Genetic Screen

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