Genetically programmed in vivo packaging of protein cargo and its controlled release from bacteriophage P22

Alison O'Neil, Courtney Reichhardt, Benjamin Johnson, Peter E. Prevelige, Trevor Douglas

Research output: Contribution to journalArticlepeer-review

143 Scopus citations

Abstract

Packed and ready to go: A scaffold protein (SP) aids the assembly of Salmonella typhimuriam bacteriophage P22 into a capsid, with encapsulation of the SP. This natural process was exploited by using an engineered molecular system to fuse a fluorescent protein cargo (green in the picture) to a portion of the SP (yellow), which templated accurate spontaneous assembly. Heating of the capsids and treatment with thrombin released the SP but not the cargo.

Original languageEnglish
Pages (from-to)7425-7428
Number of pages4
JournalAngewandte Chemie - International Edition
Volume50
Issue number32
DOIs
StatePublished - Aug 1 2011

Keywords

  • bacteriophages
  • encapsulation
  • fluorescent proteins
  • gene technology
  • nanocontainers

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