Genetically programmed in vivo packaging of protein cargo and its controlled release from bacteriophage P22

Alison O'Neil; Courtney Reichhardt; Benjamin Johnson; Peter E. Prevelige; Trevor Douglas

doi:10.1002/anie.201102036

Genetically programmed in vivo packaging of protein cargo and its controlled release from bacteriophage P22

Alison O'Neil, Courtney Reichhardt, Benjamin Johnson, Peter E. Prevelige, Trevor Douglas

Research output: Contribution to journal › Article › peer-review

133 Scopus citations

Abstract

Packed and ready to go: A scaffold protein (SP) aids the assembly of Salmonella typhimuriam bacteriophage P22 into a capsid, with encapsulation of the SP. This natural process was exploited by using an engineered molecular system to fuse a fluorescent protein cargo (green in the picture) to a portion of the SP (yellow), which templated accurate spontaneous assembly. Heating of the capsids and treatment with thrombin released the SP but not the cargo.

Original language	English
Pages (from-to)	7425-7428
Number of pages	4
Journal	Angewandte Chemie - International Edition
Volume	50
Issue number	32
DOIs	https://doi.org/10.1002/anie.201102036
State	Published - Aug 1 2011

Keywords

bacteriophages
encapsulation
fluorescent proteins
gene technology
nanocontainers

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AU - Douglas, Trevor

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KW - nanocontainers

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Genetically programmed in vivo packaging of protein cargo and its controlled release from bacteriophage P22

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