TY - JOUR
T1 - Genetically encoded calcium indicators to probe complex brain circuit dynamics in vivo
AU - Inoue, Masatoshi
N1 - Publisher Copyright:
© 2020 Elsevier B.V. and Japan Neuroscience Society
PY - 2021/8
Y1 - 2021/8
N2 - Over the past two decades, genetically encoded calcium indicators (GECIs) have been used extensively to report intracellular calcium (Ca2+) dynamics in order to readout neuronal and network activity in living tissue. Single wavelength GECIs, such as GCaMP, have been widely adapted due to advances in dynamic range, sensitivity, and kinetics. Additionally, recent efforts in protein engineering have expanded the GECI color palette to enable direct optical interrogation of more complex circuit dynamics. Here, I discuss the engineering, application, and future directions of the most recently developed GECIs for in vivo neuroscience research.
AB - Over the past two decades, genetically encoded calcium indicators (GECIs) have been used extensively to report intracellular calcium (Ca2+) dynamics in order to readout neuronal and network activity in living tissue. Single wavelength GECIs, such as GCaMP, have been widely adapted due to advances in dynamic range, sensitivity, and kinetics. Additionally, recent efforts in protein engineering have expanded the GECI color palette to enable direct optical interrogation of more complex circuit dynamics. Here, I discuss the engineering, application, and future directions of the most recently developed GECIs for in vivo neuroscience research.
KW - All-optical experiments
KW - Calcium indicator
KW - Genetically encoded fluorescent indicators
KW - Multiplex imaging
KW - Neuronal activity recording
KW - Protein engineering
KW - R-CaMP2
KW - XCaMP
UR - http://www.scopus.com/inward/record.url?scp=85086834995&partnerID=8YFLogxK
U2 - 10.1016/j.neures.2020.05.013
DO - 10.1016/j.neures.2020.05.013
M3 - Review article
C2 - 32531233
AN - SCOPUS:85086834995
SN - 0168-0102
VL - 169
SP - 2
EP - 8
JO - Neuroscience Research
JF - Neuroscience Research
ER -