Genetic mapping reveals Pou2af2/OCA-T1–dependent tuning of tuft cell differentiation and intestinal type 2 immunity

Marija S. Nadjsombati, Natalie Niepoth, Lily M. Webeck, Elizabeth A. Kennedy, Danielle L. Jones, Tyler E. Billipp, Megan T. Baldridge, Andres Bendesky, Jakob von Moltke

Research output: Contribution to journalArticlepeer-review

3 Scopus citations

Abstract

Chemosensory epithelial tuft cells contribute to innate immunity at barrier surfaces, but their differentiation from epithelial progenitors is not well understood. Here, we exploited differences between inbred mouse strains to identify an epithelium-intrinsic mechanism that regulates tuft cell differentiation and tunes innate type 2 immunity in the small intestine. Balb/cJ (Balb) mice had fewer intestinal tuft cells than C57BL/6J (B6) mice and failed to respond to the tuft cell ligand succinate. Most of this differential succinate response was determined by the 50- to 67-Mb interval of chromosome 9 (Chr9), such that congenic Balb mice carrying the B6 Chr9 interval had elevated baseline numbers of tuft cells and responded to succinate. The Chr9 locus includes Pou2af2, which encodes the protein OCA-T1, a transcriptional cofactor essential for tuft cell development. Epithelial crypts expressed a previously unannotated short isoform of Pou2af2 predicted to use a distinct transcriptional start site and encode a nonfunctional protein. Low tuft cell numbers and the resulting lack of succinate response in Balb mice were explained by a preferential expression of the short isoform and could be rescued by expression of full-length Pou2af2. Physiologically, Pou2af2 isoform usage tuned innate type 2 immunity in the small intestine. Balb mice maintained responsiveness to helminth pathogens while ignoring commensal Tritrichomonas protists and reducing norovirus burdens.

Original languageEnglish
Article numbereade5019
JournalScience immunology
Volume8
Issue number83
DOIs
StatePublished - May 2023

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