Genetic manipulation of cardiac K+ channel function in mice: What have we learned, and where do we go from here?

Jeanne M. Nerbonne, Colin G. Nichols, Thomas L. Schwarz, Denis Escande

Research output: Contribution to journalReview articlepeer-review

178 Scopus citations

Abstract

In the mammalian myocardium, potassium (K+) channels control resting potentials, action potential waveforms, automaticity, and refractory periods and, in most cardiac cells, multiple types of K+ channels that subserve these functions are expressed. Molecular cloning has revealed the presence of a large number of K+ channel pore forming (α) and accessory (β) subunits in the heart, and considerable progress has been made recently in defining the relationships between expressed K+ channel subunits and functional cardiac K+ channels. To date, more than 20 mouse models with altered K+ channel expression/functioning have been generated using dominant-negative transgenic and targeted gene deletion approaches. In several instances, the genetic manipulation of K+ channel subunit expression has revealed the role of specific K+ channel subunit subfamilies or individual K+ channel subunit genes in the generation of myocardial K+ channels. In other cases, however, the phenotypic consequences have been unexpected. This review summarizes what has been learned from the in situ genetic manipulation of cardiac K+ channel functioning in the mouse, discusses the limitations of the models developed to date, and explores the likely directions of future research.

Original languageEnglish
Pages (from-to)944-956
Number of pages13
JournalCirculation research
Volume89
Issue number11
DOIs
StatePublished - Nov 23 2001

Keywords

  • Cardiac remodeling
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  • Mouse models

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