Genetic evolution of a helicobacter pylori acid-sensing histidine kinase and gastric disease

Uma Krishna; Judith Romero-Gallo; Giovanni Suarez; Ayeetin Azah; Andrzej M. Krezel; Matthew G. Varga; Mark H. Forsyth; Richard M. Peek

doi:10.1093/infdis/jiw189

Genetic evolution of a helicobacter pylori acid-sensing histidine kinase and gastric disease

Uma Krishna, Judith Romero-Gallo, Giovanni Suarez, Ayeetin Azah, Andrzej M. Krezel, Matthew G. Varga, Mark H. Forsyth, Richard M. Peek

Department of Biochemistry & Molecular Biophysics

Research output: Contribution to journal › Article › peer-review

5 Scopus citations

Abstract

Helicobacter pylori is the strongest risk factor for gastric adenocarcinoma, which develops within a hypochlorhydric environment. We sequentially isolated H. pylori (strain J99) from a patient who developed corpus-predominant gastritis and hypochlorhydia over a 6-year interval. Archival J99 survived significantly better under acidic conditions than recent J99 strains. H. pylori arsRS encodes a 2-component system critical for stress responses; recent J99 isolates harbored 2 nonsynonymous arsS mutations, and arsS inactivation abolished acid survival. In vivo, acid-resistant archival, but not recent J99, successfully colonized high-acid-secreting rodents. Thus, genetic evolution of arsS may influence progression to hypochlorhydia and gastric cancer.

Original language	English
Pages (from-to)	644-648
Number of pages	5
Journal	Journal of Infectious Diseases
Volume	214
Issue number	4
DOIs	https://doi.org/10.1093/infdis/jiw189
State	Published - Aug 15 2016

Keywords

Acid resistance
H. pylori
Hypochlorhydia

Access to Document

10.1093/infdis/jiw189

Cite this

@article{f32c2ddefa8247e08aa3f9c1608fc6bd,

title = "Genetic evolution of a helicobacter pylori acid-sensing histidine kinase and gastric disease",

abstract = "Helicobacter pylori is the strongest risk factor for gastric adenocarcinoma, which develops within a hypochlorhydric environment. We sequentially isolated H. pylori (strain J99) from a patient who developed corpus-predominant gastritis and hypochlorhydia over a 6-year interval. Archival J99 survived significantly better under acidic conditions than recent J99 strains. H. pylori arsRS encodes a 2-component system critical for stress responses; recent J99 isolates harbored 2 nonsynonymous arsS mutations, and arsS inactivation abolished acid survival. In vivo, acid-resistant archival, but not recent J99, successfully colonized high-acid-secreting rodents. Thus, genetic evolution of arsS may influence progression to hypochlorhydia and gastric cancer.",

keywords = "Acid resistance, H. pylori, Hypochlorhydia",

author = "Uma Krishna and Judith Romero-Gallo and Giovanni Suarez and Ayeetin Azah and Krezel, {Andrzej M.} and Varga, {Matthew G.} and Forsyth, {Mark H.} and Peek, {Richard M.}",

note = "Funding Information: Financial support. This work was supported by the American Gastroenterological Association (Investing in the Future student research fellowship) and the National Institutes of Health (grants R25DK096968, R01CA077955, R01DK058587, P30DK058404, and P01CA116087). Publisher Copyright: {\textcopyright} The Author 2016.",

year = "2016",

month = aug,

day = "15",

doi = "10.1093/infdis/jiw189",

language = "English",

volume = "214",

pages = "644--648",

journal = "Journal of Infectious Diseases",

issn = "0022-1899",

number = "4",

}

Genetic evolution of a helicobacter pylori acid-sensing histidine kinase and gastric disease. / Krishna, Uma; Romero-Gallo, Judith; Suarez, Giovanni; Azah, Ayeetin; Krezel, Andrzej M.; Varga, Matthew G.; Forsyth, Mark H.; Peek, Richard M.

In: Journal of Infectious Diseases, Vol. 214, No. 4, 15.08.2016, p. 644-648.

Research output: Contribution to journal › Article › peer-review

TY - JOUR

T1 - Genetic evolution of a helicobacter pylori acid-sensing histidine kinase and gastric disease

AU - Krishna, Uma

AU - Romero-Gallo, Judith

AU - Suarez, Giovanni

AU - Azah, Ayeetin

AU - Krezel, Andrzej M.

AU - Varga, Matthew G.

AU - Forsyth, Mark H.

AU - Peek, Richard M.

N1 - Funding Information: Financial support. This work was supported by the American Gastroenterological Association (Investing in the Future student research fellowship) and the National Institutes of Health (grants R25DK096968, R01CA077955, R01DK058587, P30DK058404, and P01CA116087). Publisher Copyright: © The Author 2016.

PY - 2016/8/15

Y1 - 2016/8/15

N2 - Helicobacter pylori is the strongest risk factor for gastric adenocarcinoma, which develops within a hypochlorhydric environment. We sequentially isolated H. pylori (strain J99) from a patient who developed corpus-predominant gastritis and hypochlorhydia over a 6-year interval. Archival J99 survived significantly better under acidic conditions than recent J99 strains. H. pylori arsRS encodes a 2-component system critical for stress responses; recent J99 isolates harbored 2 nonsynonymous arsS mutations, and arsS inactivation abolished acid survival. In vivo, acid-resistant archival, but not recent J99, successfully colonized high-acid-secreting rodents. Thus, genetic evolution of arsS may influence progression to hypochlorhydia and gastric cancer.

AB - Helicobacter pylori is the strongest risk factor for gastric adenocarcinoma, which develops within a hypochlorhydric environment. We sequentially isolated H. pylori (strain J99) from a patient who developed corpus-predominant gastritis and hypochlorhydia over a 6-year interval. Archival J99 survived significantly better under acidic conditions than recent J99 strains. H. pylori arsRS encodes a 2-component system critical for stress responses; recent J99 isolates harbored 2 nonsynonymous arsS mutations, and arsS inactivation abolished acid survival. In vivo, acid-resistant archival, but not recent J99, successfully colonized high-acid-secreting rodents. Thus, genetic evolution of arsS may influence progression to hypochlorhydia and gastric cancer.

KW - Acid resistance

KW - H. pylori

KW - Hypochlorhydia

UR - http://www.scopus.com/inward/record.url?scp=84988952597&partnerID=8YFLogxK

U2 - 10.1093/infdis/jiw189

DO - 10.1093/infdis/jiw189

M3 - Article

C2 - 27190191

AN - SCOPUS:84988952597

VL - 214

SP - 644

EP - 648

JO - Journal of Infectious Diseases

JF - Journal of Infectious Diseases

SN - 0022-1899

IS - 4

ER -

Genetic evolution of a helicobacter pylori acid-sensing histidine kinase and gastric disease

Abstract

Keywords

Access to Document

Other files and links

Fingerprint

Cite this