Genetic evidence supporting selection of the Vα14i NKT cell lineage from double-positive thymocyte precursors

Takeshi Egawa, Gerard Eberl, Ichiro Taniuchi, Kamel Benlagha, Frederic Geissmann, Lothar Hennighausen, Albert Bendelac, Dan R. Littman

Research output: Contribution to journalArticlepeer-review

211 Scopus citations

Abstract

Invariant Vα14i NKT (iNKT) cells are a specialized subset of T lymphocytes with regulatory functions. They coexpress TCRαβ and natural killer cell markers. They differentiate through interaction of their Vα14-Jα18 invariant TCRα chains with CD1d expressed on double-positive (DP) thymocytes. Although their development has been shown to be thymus dependent, their developmental pathway has not been definitively established. By using genetic analyses, we show here that all iNKT cells are selected from a pool of DP thymocytes. Their development is absolutely dependent on Runx1 and RORγt, transcription factors that influence, but are not required for, development of conventional T cells. Our results indicate that even though CD1d binding DP thymocytes have yet to be observed, Vα14-Jα18 rearrangement in these cells is required for development of iNKT cells.

Original languageEnglish
Pages (from-to)705-716
Number of pages12
JournalImmunity
Volume22
Issue number6
DOIs
StatePublished - Jun 2005

Fingerprint

Dive into the research topics of 'Genetic evidence supporting selection of the Vα14i NKT cell lineage from double-positive thymocyte precursors'. Together they form a unique fingerprint.

Cite this