Genetic and epigenetic regulation of tcrb gene assembly

Michael L. Sikes, Eugene M. Oltz

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Scopus citations

Abstract

Vertebrate development requires the formation of multiple cell types from a single genetic blueprint, an extraordinary feat that is guided by the dynamic and finely tuned reprogramming of gene expression. The sophisticated orchestration of gene expression programs is driven primarily by changes in the patterns of covalent chromatin modifications. These epigenetic changes are directed by cis elements, positioned across the genome, which provide docking sites for transcription factors and associated chromatin modifiers. Epigenetic changes impact all aspects of gene regulation, governing association with the machinery that drives transcription, replication, repair and recombination, a regulatory relationship that is dramatically illustrated in developing lymphocytes. The program of somatic rearrangements that assemble antigen receptor genes in precursor B and T cells has proven to be a fertile system for elucidating relationships between the genetic and epigenetic components of gene regulation. This chapter describes our current understanding of the cross-talk between key genetic elements and epigenetic programs during recombination of the Tcrb locus in developing T cells, how each contributes to the regulation of chromatin accessibility at individual DNA targets for recombination, and potential mechanisms that coordinate their actions.

Original languageEnglish
Title of host publicationEpigenetic Regulation of Lymphocyte Development
EditorsCornelis Murre
Pages91-116
Number of pages26
DOIs
StatePublished - Mar 7 2012

Publication series

NameCurrent Topics in Microbiology and Immunology
Volume356
ISSN (Print)0070-217X

Keywords

  • Chromatin
  • T cell receptor genes
  • Thymocytes
  • Transcription
  • V(D)J recombination

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    Sikes, M. L., & Oltz, E. M. (2012). Genetic and epigenetic regulation of tcrb gene assembly. In C. Murre (Ed.), Epigenetic Regulation of Lymphocyte Development (pp. 91-116). (Current Topics in Microbiology and Immunology; Vol. 356). https://doi.org/10.1007/82_2011_138