Genetic analysis of glutamate receptors in drosophila reveals a retrograde signal regulating presynaptic transmitter release

Sophie A. Petersen, Richard D. Fetter, Jasprina N. Noordermeer, Corey S. Goodman, Aaron DiAntonio

Research output: Contribution to journalArticlepeer-review

360 Scopus citations

Abstract

Postsynaptic sensitivity to glutamate was genetically manipulated at the Drosophila neuromuscular junction (NMJ) to test whether postsynaptic activity can regulate presynaptic function during development. We cloned the gene encoding a second muscle-specific glutamate receptor, DGluRIIB, which is closely related to the previously identified DGluRIIA and located adjacent to it in the genome. Mutations that eliminate DGluRIIA (but not DGluRIIB) or transgenic constructs that increase DGluRIIA expression were generated. When DGluRIIA is missing, the response of the muscle to a single vesicle of transmitter is substantially decreased. However, the response of the muscle to nerve stimulation is normal because quantal content is significantly increased. Thus, a decrease in postsynaptic receptors leads to an increase in presynaptic transmitter release, indicating that postsynaptic activity controls a retrograde signal that regulates presynaptic function.

Original languageEnglish
Pages (from-to)1237-1248
Number of pages12
JournalNeuron
Volume19
Issue number6
DOIs
StatePublished - Dec 1997

Fingerprint

Dive into the research topics of 'Genetic analysis of glutamate receptors in drosophila reveals a retrograde signal regulating presynaptic transmitter release'. Together they form a unique fingerprint.

Cite this