Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations

Tina Tianbo Zhang; Shane Rui Zhao; Christina Alamana; Mengcheng Shen; Victoria Parikh; Matthew T. Wheeler; Joseph C. Wu

doi:10.1016/j.scr.2022.102941

Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations

Tina Tianbo Zhang
, Shane Rui Zhao
, Christina Alamana
, Mengcheng Shen
, Victoria Parikh
, Matthew T. Wheeler
, Joseph C. Wu

Division of Cardiology

Research output: Contribution to journal › Article › peer-review

Abstract

Dilated cardiomyopathy (DCM) is a common heart disease that can lead to heart failure and sudden cardiac death. Mutations in the TTN gene are the most frequent cause of DCM. Here, we generated two human induced pluripotent stem cell (iPSC) lines from the peripheral blood mononuclear cells (PBMCs) of two DCM patients carrying c.94816C>T and c.104188A>G mutations in TTN, respectively. The two lines exhibited a normal morphology, full expression of pluripotency markers, a normal karyotype and the ability of trilineage differentiation. The two lines can serve as useful tools for drug screening and mechanism studies on DCM.

Original language	English
Article number	102941
Journal	Stem Cell Research
Volume	65
DOIs	https://doi.org/10.1016/j.scr.2022.102941
State	Published - Dec 2022

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title = "Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations",

abstract = "Dilated cardiomyopathy (DCM) is a common heart disease that can lead to heart failure and sudden cardiac death. Mutations in the TTN gene are the most frequent cause of DCM. Here, we generated two human induced pluripotent stem cell (iPSC) lines from the peripheral blood mononuclear cells (PBMCs) of two DCM patients carrying c.94816C>T and c.104188A>G mutations in TTN, respectively. The two lines exhibited a normal morphology, full expression of pluripotency markers, a normal karyotype and the ability of trilineage differentiation. The two lines can serve as useful tools for drug screening and mechanism studies on DCM.",

author = "Zhang, \{Tina Tianbo\} and Zhao, \{Shane Rui\} and Christina Alamana and Mengcheng Shen and Victoria Parikh and Wheeler, \{Matthew T.\} and Wu, \{Joseph C.\}",

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T1 - Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations

AU - Zhang, Tina Tianbo

AU - Zhao, Shane Rui

AU - Alamana, Christina

AU - Shen, Mengcheng

AU - Parikh, Victoria

AU - Wheeler, Matthew T.

AU - Wu, Joseph C.

PY - 2022/12

Y1 - 2022/12

N2 - Dilated cardiomyopathy (DCM) is a common heart disease that can lead to heart failure and sudden cardiac death. Mutations in the TTN gene are the most frequent cause of DCM. Here, we generated two human induced pluripotent stem cell (iPSC) lines from the peripheral blood mononuclear cells (PBMCs) of two DCM patients carrying c.94816C>T and c.104188A>G mutations in TTN, respectively. The two lines exhibited a normal morphology, full expression of pluripotency markers, a normal karyotype and the ability of trilineage differentiation. The two lines can serve as useful tools for drug screening and mechanism studies on DCM.

AB - Dilated cardiomyopathy (DCM) is a common heart disease that can lead to heart failure and sudden cardiac death. Mutations in the TTN gene are the most frequent cause of DCM. Here, we generated two human induced pluripotent stem cell (iPSC) lines from the peripheral blood mononuclear cells (PBMCs) of two DCM patients carrying c.94816C>T and c.104188A>G mutations in TTN, respectively. The two lines exhibited a normal morphology, full expression of pluripotency markers, a normal karyotype and the ability of trilineage differentiation. The two lines can serve as useful tools for drug screening and mechanism studies on DCM.

UR - https://www.scopus.com/pages/publications/85140273762

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DO - 10.1016/j.scr.2022.102941

M3 - Article

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AN - SCOPUS:85140273762

SN - 1873-5061

VL - 65

JO - Stem Cell Research

JF - Stem Cell Research

M1 - 102941

ER -

Generation of two induced pluripotent stem cell lines from dilated cardiomyopathy patients carrying TTN mutations

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