TY - JOUR
T1 - Generation of iPSC line from desmin-related cardiomyopathy patient carrying splice site mutation of DES gene
AU - Khudiakov, Aleksandr
AU - Kostina, Daria
AU - Zlotina, Anna
AU - Nikulina, Tatiana
AU - Sergushichev, Alexey
AU - Gudkova, Alexandra
AU - Tomilin, Alexey
AU - Malashicheva, Anna
AU - Kostareva, Anna
N1 - Publisher Copyright:
© 2017
PY - 2017/10
Y1 - 2017/10
N2 - Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying desmin (DES) gene heterozygous splice site mutation using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. iPSCs were characterized by sequencing, karyotype analysis, STR analysis, immunocytochemistry, RT-PCR and teratoma formation.
AB - Human iPSC line was generated from patient-specific adipose tissue-derived mesenchymal multipotent stromal cells carrying desmin (DES) gene heterozygous splice site mutation using non-integrative reprogramming method. Reprogramming factors OCT4, KLF4, SOX2, CMYC were delivered using Sendai viruses. iPSCs were characterized by sequencing, karyotype analysis, STR analysis, immunocytochemistry, RT-PCR and teratoma formation.
UR - http://www.scopus.com/inward/record.url?scp=85027990665&partnerID=8YFLogxK
U2 - 10.1016/j.scr.2017.08.015
DO - 10.1016/j.scr.2017.08.015
M3 - Article
C2 - 29034897
AN - SCOPUS:85027990665
SN - 1873-5061
VL - 24
SP - 77
EP - 80
JO - Stem Cell Research
JF - Stem Cell Research
ER -