@inbook{d044ee01395649b59877c343fa83a6cc,
title = "Generation of Human Neurons by microRNA-Mediated Direct Conversion of Dermal Fibroblasts",
abstract = "MicroRNAs (miRNAs), miR-9/9*, and miR-124 (miR-9/9*-124) display fate-reprogramming activities when ectopically expressed in human fibroblasts by erasing the fibroblast identity and evoking a pan-neuronal state. In contrast to induced pluripotent stem cell-derived neurons, miRNA-induced neurons (miNs) retain the biological age of the starting fibroblasts through direct fate conversion and thus provide a human neuron-based platform to study cellular properties inherent in aged neurons and model adult-onset neurodegenerative disorders using patient-derived cells. Furthermore, expression of neuronal subtype-specific transcription factors in conjunction with miR-9/9*-124 guides the miNs to distinct neuronal fates, a feature critical for modeling disorders that affect specific neuronal subtypes. Here, we describe the miR-9/9*-124-based neuronal reprogramming protocols for the generation of several disease-relevant neuronal subtypes: striatal medium spiny neurons, cortical neurons, and spinal cord motor neurons.",
keywords = "Aging, Cortical neuron, Human neuron, Medium spiny neuron, Motor neuron, Neurogenesis, Neuronal cell-fate, Neuronal reprogramming, miN, miRNA-mediated direct conversion",
author = "Church, {Victoria A.} and Kitra Cates and Lucia Capano and Shivani Aryal and Kim, {Woo Kyung} and Yoo, {Andrew S.}",
note = "Funding Information: The authors thank Shawei Chen for his inputs related to the protocol. V.A.C. is supported by a fellowship from the William N. and Bernice E. Bumpus Foundation. This work was supported by grants from NIH (RF1AG056296 and R01NS107488), CHDI foundation, Farrell Fund, and Collaborative Center for X-linked Dystonia Parkinsonism (CC-XDP) to A.S.Y. Publisher Copyright: {\textcopyright} 2021, Springer Science+Business Media, LLC, part of Springer Nature.",
year = "2021",
doi = "10.1007/978-1-0716-1084-8_6",
language = "English",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "77--100",
booktitle = "Methods in Molecular Biology",
}