Generation of gene-specific mutated rats using zinc-finger nucleases.

Aron M. Geurts, Gregory J. Cost, Séverine Rémy, Xiaoxia Cui, Laurent Tesson, Claire Usal, Séverine Ménoret, Howard J. Jacob, Ignacio Anegon, Roland Buelow

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Abstract

The genetic dissection of physiological and pathological traits in laboratory model organisms is accelerated by the ability to engineer loss-of-function mutations at investigator-specified loci. This chapter describes the use of zinc-finger nucleases (ZFNs) for the targeted disruption of endogenous rat genes directly in the embryo. ZFNs can specifically disrupt target genes in cultured rat cells and in embryos from inbred and outbred strains, leading to permanently genetically modified animals. This technology allows for the rapid, targeted modification of the rat genome.

Original languageEnglish
Pages (from-to)211-225
Number of pages15
JournalMethods in molecular biology (Clifton, N.J.)
Volume597
StatePublished - 2010
Externally publishedYes

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    Geurts, A. M., Cost, G. J., Rémy, S., Cui, X., Tesson, L., Usal, C., Ménoret, S., Jacob, H. J., Anegon, I., & Buelow, R. (2010). Generation of gene-specific mutated rats using zinc-finger nucleases. Methods in molecular biology (Clifton, N.J.), 597, 211-225.