Generation of an adenoviral vector containing an addition of a heterologous ligand to the serotype 3 fiber knob

Taco G. Uil, Toshiro Seki, Igor Dmitriev, Elena Kashentseva, Joanne T. Douglas, Marianne G. Rots, Jaap M. Middeldorp, David T. Curiel

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

As an initial assessment of the feasibility of employing the adenovirus serotype 3 (Ad3) fiber knob as a locale for introducing a tropism-modifying motif, we generated an adenoviral vector containing a six-histidine tag genetically fused to the carboxy-terminus of the Ad3 fiber knob. The heterologous tag proved to be accessible for binding in the context of the virion and, moreover, had rendered the modified vector capable of mediating gene transfer through an artificial, non-Ad3 receptor.

Original languageEnglish
Pages (from-to)121-124
Number of pages4
JournalCancer gene therapy
Volume10
Issue number2
DOIs
StatePublished - Feb 1 2003

Keywords

  • Ad3 knob
  • Capsid modification
  • Knob chimerism
  • Targeting
  • Tropism

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