Generation of allospecffic natural killer cells by stimulation across a polymorphism of HLA-C

Marco Colonna; Edward G. Brooks; Michela Falco; Giovan Battista Ferrara; Jack L. Strominger

Generation of allospecffic natural killer cells by stimulation across a polymorphism of HLA-C

Marco Colonna, Edward G. Brooks, Michela Falco, Giovan Battista Ferrara, Jack L. Strominger

Research output: Contribution to journal › Article › peer-review

Abstract

The cytotoxicity of human natural killer (NK) cells is modulated by the major histocom-patibility complex human leukocyte antigen (HLA)-C molecules on the surface of the target cell. Alloreactive NK cells specific for the NK-1 alloantigen could be reproducibly generated from individuals that were homozygous for HLA-C with asparagine at residue 77 and lysine at residue 80 [HLA-CfAsn⁷⁷, Lys⁸⁰)] by stimulation with target cells that were homozygous for HLA-C(Ser⁷⁷, Asn⁸⁰); the reciprocal stimulation yielded NK cells specific for the NK-2 alloantigen. However, neither homozygous target cell stimulated the generation of alloreactive NK cells from heterozygous individuals. Thus, these data reveal an unanticipated difference between human NK alloreactivity defined by this system and murine "hybrid resistance.".

Original language	English
Pages (from-to)	1121-1124
Number of pages	4
Journal	Science
Volume	260
Issue number	5111
State	Published - 1993

Cite this

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title = "Generation of allospecffic natural killer cells by stimulation across a polymorphism of HLA-C",

abstract = "The cytotoxicity of human natural killer (NK) cells is modulated by the major histocom-patibility complex human leukocyte antigen (HLA)-C molecules on the surface of the target cell. Alloreactive NK cells specific for the NK-1 alloantigen could be reproducibly generated from individuals that were homozygous for HLA-C with asparagine at residue 77 and lysine at residue 80 [HLA-CfAsn77, Lys80)] by stimulation with target cells that were homozygous for HLA-C(Ser77, Asn80); the reciprocal stimulation yielded NK cells specific for the NK-2 alloantigen. However, neither homozygous target cell stimulated the generation of alloreactive NK cells from heterozygous individuals. Thus, these data reveal an unanticipated difference between human NK alloreactivity defined by this system and murine {"}hybrid resistance.{"}.",

author = "Marco Colonna and Brooks, {Edward G.} and Michela Falco and Ferrara, {Giovan Battista} and Strominger, {Jack L.}",

year = "1993",

language = "English",

volume = "260",

pages = "1121--1124",

journal = "Science",

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TY - JOUR

T1 - Generation of allospecffic natural killer cells by stimulation across a polymorphism of HLA-C

AU - Colonna, Marco

AU - Brooks, Edward G.

AU - Falco, Michela

AU - Ferrara, Giovan Battista

AU - Strominger, Jack L.

PY - 1993

Y1 - 1993

N2 - The cytotoxicity of human natural killer (NK) cells is modulated by the major histocom-patibility complex human leukocyte antigen (HLA)-C molecules on the surface of the target cell. Alloreactive NK cells specific for the NK-1 alloantigen could be reproducibly generated from individuals that were homozygous for HLA-C with asparagine at residue 77 and lysine at residue 80 [HLA-CfAsn77, Lys80)] by stimulation with target cells that were homozygous for HLA-C(Ser77, Asn80); the reciprocal stimulation yielded NK cells specific for the NK-2 alloantigen. However, neither homozygous target cell stimulated the generation of alloreactive NK cells from heterozygous individuals. Thus, these data reveal an unanticipated difference between human NK alloreactivity defined by this system and murine "hybrid resistance.".

AB - The cytotoxicity of human natural killer (NK) cells is modulated by the major histocom-patibility complex human leukocyte antigen (HLA)-C molecules on the surface of the target cell. Alloreactive NK cells specific for the NK-1 alloantigen could be reproducibly generated from individuals that were homozygous for HLA-C with asparagine at residue 77 and lysine at residue 80 [HLA-CfAsn77, Lys80)] by stimulation with target cells that were homozygous for HLA-C(Ser77, Asn80); the reciprocal stimulation yielded NK cells specific for the NK-2 alloantigen. However, neither homozygous target cell stimulated the generation of alloreactive NK cells from heterozygous individuals. Thus, these data reveal an unanticipated difference between human NK alloreactivity defined by this system and murine "hybrid resistance.".

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M3 - Article

C2 - 8493555

AN - SCOPUS:0027175363

SN - 0036-8075

VL - 260

SP - 1121

EP - 1124

JO - Science

JF - Science

IS - 5111

ER -

Generation of allospecffic natural killer cells by stimulation across a polymorphism of HLA-C

Abstract

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