Generation of a transgenic mouse model with chondrocyte-specific and tamoxifen-inducible expression of Cre recombinase

Mo Chen, Alexander C. Lichtler, Tzong Jen Sheu, Chao Xie, Xinping Zhang, Regis J. O'Keefe, Di Chen

Research output: Contribution to journalArticle

95 Scopus citations

Abstract

Postnatal cartilage development and growth are regulated by key growth factors and signaling molecules. To fully understand the function of these regulators, an inducible and chondrocyte-specific gene deletion system needs to be established to circumvent the perinatal lethality. In this report, we have generated a transgenic mouse model (Col2a1-CreERT2) in which expression of the Cre recombinase is driven by the chondrocyte-specific col2a1 promoter in a tamoxifen-inducible manner. To determine the specificity and efficiency of the Cre recombination, we have bred Col2a1-CreERT2 mice with Rosa26R reporter mice. The X-Gal staining showed that the Cre recombination is specifically achieved in cartilage tissues with tamoxifen-induction. In vitro experiments of chondrocyte cell culture also demonstrate the 4-hydroxy tamoxifen-induced Cre recombination. These results demonstrate that Col2a1-CreERT2 transgenic mice can be used as a valuable tool for an inducible and chondrocyte-specific gene deletion approach.

Original languageEnglish
Pages (from-to)44-50
Number of pages7
JournalGenesis
Volume45
Issue number1
DOIs
StatePublished - Jan 1 2007
Externally publishedYes

Keywords

  • Chondrocyte
  • Conditional knockout
  • Cre-mediated recombination
  • Tamoxifen
  • X-gal staining

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