Generation and characterization of the PKCγ-Cre mouse line

Yu Qiang Ding, Chuan Xi Xiang, Zhou Feng Chen

Research output: Contribution to journalArticle

9 Scopus citations

Abstract

PKCγ is a protein kinase C (PKC) isoform that is expressed in the central nervous system (CNS). We generated a PKCγ-Cre mouse line and characterized the expression and activity of the Cre protein. Our studies show that Cre expression largely recapitulates the endogenous expression of PKCγ. Several major sites of Cre expression are the cerebral cortex, hippocampus, thalamus, amygdala, and spinal cord. Examination of PKCγ-Cre/ROSA26 mice reveals a similar X-gal staining pattern in the CNS, indicating that Cre recombinase is capable of removing LoxP sites in vivo. These data indicate that the PKCγ-Cre mouse line could be a useful reagent for generating Cre-mediated tissue-specific knockouts in the CNS.

Original languageEnglish
Pages (from-to)28-33
Number of pages6
JournalGenesis
Volume43
Issue number1
DOIs
StatePublished - Sep 1 2005

Keywords

  • Brain
  • Cre
  • Gene targeting
  • PKCγ
  • Spinal cord

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