Here we present a protocol for generating transgenic embryos in Xenopus laevis and Xenopus tropicalis. The method includes three steps: (1) The preparation of high-speed egg extracts, which facilitates the replacement of protamines in sperm nuclei with nucleosomes and decondenses the chromatin of sperm nuclei; (2) The isolation of sperm nuclei; and (3) The mixing of sperm nuclei, restriction enzyme, and high-speed extract in vitro, following by nuclear transplantation into unfertilized eggs to generate the transgenic embryos. This procedure generates non-mosaic transgenic embryos at high frequency and efficiency.

Original languageEnglish
Title of host publicationXenopus Protocols
Subtitle of host publicationPost-Genomic Approaches
PublisherHumana Press Inc.
Number of pages19
ISBN (Print)9781617799914
StatePublished - 2012

Publication series

NameMethods in Molecular Biology
ISSN (Print)1064-3745


  • Restriction enzyme mediated integration
  • Transgenesis
  • Xenopus


Dive into the research topics of 'Generating transgenic frog embryos by Restriction Enzyme Mediated Integration (REMI)'. Together they form a unique fingerprint.

Cite this