@inbook{420ce80aa6054c939fe3fc0502349404,
title = "Generating transgenic frog embryos by Restriction Enzyme Mediated Integration (REMI)",
abstract = "Here we present a protocol for generating transgenic embryos in Xenopus laevis and Xenopus tropicalis. The method includes three steps: (1) The preparation of high-speed egg extracts, which facilitates the replacement of protamines in sperm nuclei with nucleosomes and decondenses the chromatin of sperm nuclei; (2) The isolation of sperm nuclei; and (3) The mixing of sperm nuclei, restriction enzyme, and high-speed extract in vitro, following by nuclear transplantation into unfertilized eggs to generate the transgenic embryos. This procedure generates non-mosaic transgenic embryos at high frequency and efficiency.",
keywords = "Restriction enzyme mediated integration, Transgenesis, Xenopus",
author = "Shoko Ishibashi and Kroll, {Kristen L.} and Enrique Amaya",
year = "2012",
doi = "10.1007/978-1-61779-992-1_11",
language = "English",
isbn = "9781617799914",
series = "Methods in Molecular Biology",
publisher = "Humana Press Inc.",
pages = "185--203",
booktitle = "Xenopus Protocols",
}