Gene expression profiling of breast cancer in Lebanese women

Joelle Makoukji; Nadine J. Makhoul; Maya Khalil; Sally El-Sitt; Ehab Saad Aldin; Mark Jabbour; Fouad Boulos; Emanuela Gadaleta; Ajanthah Sangaralingam; Claude Chelala; Rose Mary Boustany; Arafat Tfayli

doi:10.1038/srep36639

Gene expression profiling of breast cancer in Lebanese women

Joelle Makoukji, Nadine J. Makhoul, Maya Khalil, Sally El-Sitt, Ehab Saad Aldin, Mark Jabbour, Fouad Boulos, Emanuela Gadaleta, Ajanthah Sangaralingam, Claude Chelala, Rose Mary Boustany, Arafat Tfayli

Research output: Contribution to journal › Article › peer-review

35 Scopus citations

Abstract

Breast cancer is commonest cancer in women worldwide. Elucidation of underlying biology and molecular pathways is necessary for improving therapeutic options and clinical outcomes. Molecular alterations in breast cancer are complex and involve cross-talk between multiple signaling pathways. The aim of this study is to extract a unique mRNA fingerprint of breast cancer in Lebanese women using microarray technologies. Gene-expression profiles of 94 fresh breast tissue samples (84 cancerous/10 non-tumor adjacent samples) were analyzed using GeneChip Human Genome U133 Plus 2.0 arrays. Quantitative real-time PCR was employed to validate candidate genes. Differentially expressed genes between breast cancer and non-tumor tissues were screened. Significant differences in gene expression were established for COL11A1/COL10A1/MMP1/COL6A6/DLK1/S100P/CXCL11/SOX11/LEP/ADIPOQ/OXTR/FOSL1/ACSBG1 and C21orf37. Pathways/diseases representing these genes were retrieved and linked using PANTHER ®/Pathway Studio ®. Many of the deregulated genes are associated with extracellular matrix, inflammation, angiogenesis, metastasis, differentiation, cell proliferation and tumorigenesis. Characteristics of breast cancers in Lebanese were compared to those of women from Western populations to explain why breast cancer is more aggressive and presents a decade earlier in Lebanese victims. Delineating molecular mechanisms of breast cancer in Lebanese women led to key genes which could serve as potential biomarkers and/or novel drug targets for breast cancer.

Original language	English
Article number	36639
Journal	Scientific reports
Volume	6
DOIs	https://doi.org/10.1038/srep36639
State	Published - Nov 18 2016

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title = "Gene expression profiling of breast cancer in Lebanese women",

abstract = "Breast cancer is commonest cancer in women worldwide. Elucidation of underlying biology and molecular pathways is necessary for improving therapeutic options and clinical outcomes. Molecular alterations in breast cancer are complex and involve cross-talk between multiple signaling pathways. The aim of this study is to extract a unique mRNA fingerprint of breast cancer in Lebanese women using microarray technologies. Gene-expression profiles of 94 fresh breast tissue samples (84 cancerous/10 non-tumor adjacent samples) were analyzed using GeneChip Human Genome U133 Plus 2.0 arrays. Quantitative real-time PCR was employed to validate candidate genes. Differentially expressed genes between breast cancer and non-tumor tissues were screened. Significant differences in gene expression were established for COL11A1/COL10A1/MMP1/COL6A6/DLK1/S100P/CXCL11/SOX11/LEP/ADIPOQ/OXTR/FOSL1/ACSBG1 and C21orf37. Pathways/diseases representing these genes were retrieved and linked using PANTHER {\textregistered}/Pathway Studio {\textregistered}. Many of the deregulated genes are associated with extracellular matrix, inflammation, angiogenesis, metastasis, differentiation, cell proliferation and tumorigenesis. Characteristics of breast cancers in Lebanese were compared to those of women from Western populations to explain why breast cancer is more aggressive and presents a decade earlier in Lebanese victims. Delineating molecular mechanisms of breast cancer in Lebanese women led to key genes which could serve as potential biomarkers and/or novel drug targets for breast cancer.",

author = "Joelle Makoukji and Makhoul, {Nadine J.} and Maya Khalil and Sally El-Sitt and Aldin, {Ehab Saad} and Mark Jabbour and Fouad Boulos and Emanuela Gadaleta and Ajanthah Sangaralingam and Claude Chelala and Boustany, {Rose Mary} and Arafat Tfayli",

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AU - Jabbour, Mark

AU - Boulos, Fouad

AU - Gadaleta, Emanuela

AU - Sangaralingam, Ajanthah

AU - Chelala, Claude

AU - Boustany, Rose Mary

AU - Tfayli, Arafat

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AB - Breast cancer is commonest cancer in women worldwide. Elucidation of underlying biology and molecular pathways is necessary for improving therapeutic options and clinical outcomes. Molecular alterations in breast cancer are complex and involve cross-talk between multiple signaling pathways. The aim of this study is to extract a unique mRNA fingerprint of breast cancer in Lebanese women using microarray technologies. Gene-expression profiles of 94 fresh breast tissue samples (84 cancerous/10 non-tumor adjacent samples) were analyzed using GeneChip Human Genome U133 Plus 2.0 arrays. Quantitative real-time PCR was employed to validate candidate genes. Differentially expressed genes between breast cancer and non-tumor tissues were screened. Significant differences in gene expression were established for COL11A1/COL10A1/MMP1/COL6A6/DLK1/S100P/CXCL11/SOX11/LEP/ADIPOQ/OXTR/FOSL1/ACSBG1 and C21orf37. Pathways/diseases representing these genes were retrieved and linked using PANTHER ®/Pathway Studio ®. Many of the deregulated genes are associated with extracellular matrix, inflammation, angiogenesis, metastasis, differentiation, cell proliferation and tumorigenesis. Characteristics of breast cancers in Lebanese were compared to those of women from Western populations to explain why breast cancer is more aggressive and presents a decade earlier in Lebanese victims. Delineating molecular mechanisms of breast cancer in Lebanese women led to key genes which could serve as potential biomarkers and/or novel drug targets for breast cancer.

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Gene expression profiling of breast cancer in Lebanese women

Abstract

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