Gene expression profiling: Monitoring transcription and translation products using DNA microarrays and proteomics

Julio E. Celis; Mogens Kruhøffer; Irina Gromova; Casper Frederiksen; Morten Østergaard; Thomas Thykjaer; Pavel Gromov; Jinsheng Yu; Hildur Pálsdóttir; Nils Magnusson; Torben F. Ørntoft

doi:10.1016/S0014-5793(00)01771-3

Gene expression profiling: Monitoring transcription and translation products using DNA microarrays and proteomics

Julio E. Celis, Mogens Kruhøffer, Irina Gromova, Casper Frederiksen, Morten Østergaard, Thomas Thykjaer, Pavel Gromov, Jinsheng Yu, Hildur Pálsdóttir, Nils Magnusson, Torben F. Ørntoft

Genome Technology Access Center (GTAC)

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268 Scopus citations

Abstract

Novel and powerful technologies such as DNA microarrays and proteomics have made possible the analysis of the expression levels of multiple genes simultaneously both in health and disease. In combination, these technologies promise to revolutionize biology, in particular in the area of molecular medicine as they are expected to reveal gene regulation events involved in disease progression as well as to pinpoint potential targets for drug discovery and diagnostics. Here, we review the current status of these technologies and highlight some studies in which they have been applied in concert to the analysis of biopsy specimens. (C) 2000 Federation of European Biochemical Societies.

Original language	English
Pages (from-to)	2-16
Number of pages	15
Journal	FEBS Letters
Volume	480
Issue number	1
DOIs	https://doi.org/10.1016/S0014-5793(00)01771-3
State	Published - Aug 25 2000

Keywords

Bladder cancer
Gene expression profile
Immunohistochemistry
Microarray
Proteome
Two-dimensional gel electrophoresis

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10.1016/S0014-5793(00)01771-3

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title = "Gene expression profiling: Monitoring transcription and translation products using DNA microarrays and proteomics",

abstract = "Novel and powerful technologies such as DNA microarrays and proteomics have made possible the analysis of the expression levels of multiple genes simultaneously both in health and disease. In combination, these technologies promise to revolutionize biology, in particular in the area of molecular medicine as they are expected to reveal gene regulation events involved in disease progression as well as to pinpoint potential targets for drug discovery and diagnostics. Here, we review the current status of these technologies and highlight some studies in which they have been applied in concert to the analysis of biopsy specimens. (C) 2000 Federation of European Biochemical Societies.",

keywords = "Bladder cancer, Gene expression profile, Immunohistochemistry, Microarray, Proteome, Two-dimensional gel electrophoresis",

author = "Celis, {Julio E.} and Mogens Kruh{\o}ffer and Irina Gromova and Casper Frederiksen and Morten {\O}stergaard and Thomas Thykjaer and Pavel Gromov and Jinsheng Yu and Hildur P{\'a}lsd{\'o}ttir and Nils Magnusson and {\O}rntoft, {Torben F.}",

note = "Funding Information: We would like to thank H. Wolf for many stimulating discussions. This work was supported by grants from the Danish Cancer Society, the Danish Biotechnology Programme, the John and Birthe Meyer Foundation and the Molecular Gerontology Centre.",

year = "2000",

month = aug,

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doi = "10.1016/S0014-5793(00)01771-3",

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T2 - Monitoring transcription and translation products using DNA microarrays and proteomics

AU - Celis, Julio E.

AU - Kruhøffer, Mogens

AU - Gromova, Irina

AU - Frederiksen, Casper

AU - Østergaard, Morten

AU - Thykjaer, Thomas

AU - Gromov, Pavel

AU - Yu, Jinsheng

AU - Pálsdóttir, Hildur

AU - Magnusson, Nils

AU - Ørntoft, Torben F.

N1 - Funding Information: We would like to thank H. Wolf for many stimulating discussions. This work was supported by grants from the Danish Cancer Society, the Danish Biotechnology Programme, the John and Birthe Meyer Foundation and the Molecular Gerontology Centre.

PY - 2000/8/25

Y1 - 2000/8/25

N2 - Novel and powerful technologies such as DNA microarrays and proteomics have made possible the analysis of the expression levels of multiple genes simultaneously both in health and disease. In combination, these technologies promise to revolutionize biology, in particular in the area of molecular medicine as they are expected to reveal gene regulation events involved in disease progression as well as to pinpoint potential targets for drug discovery and diagnostics. Here, we review the current status of these technologies and highlight some studies in which they have been applied in concert to the analysis of biopsy specimens. (C) 2000 Federation of European Biochemical Societies.

AB - Novel and powerful technologies such as DNA microarrays and proteomics have made possible the analysis of the expression levels of multiple genes simultaneously both in health and disease. In combination, these technologies promise to revolutionize biology, in particular in the area of molecular medicine as they are expected to reveal gene regulation events involved in disease progression as well as to pinpoint potential targets for drug discovery and diagnostics. Here, we review the current status of these technologies and highlight some studies in which they have been applied in concert to the analysis of biopsy specimens. (C) 2000 Federation of European Biochemical Societies.

KW - Bladder cancer

KW - Gene expression profile

KW - Immunohistochemistry

KW - Microarray

KW - Proteome

KW - Two-dimensional gel electrophoresis

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DO - 10.1016/S0014-5793(00)01771-3

M3 - Review article

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JF - FEBS Letters

IS - 1

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Gene expression profiling: Monitoring transcription and translation products using DNA microarrays and proteomics

Abstract

Keywords

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