Gel shift assay of nuclear extracts from Histoplasma capsulatum demonstrates the presence of several DNA binding proteins

Atanas Ignatov, Elizabeth J. Keath

Research output: Contribution to journalArticle

6 Scopus citations

Abstract

A gel shift assay was optimized to detect several general DNA binding proteins from Histoplasma capsulatum strain G217B. The electrophoretic mobility shift assay (EMSA) technique also detected protein(s) recognizing a pyrimidine-rich motif found in several Histoplasma promoters. Establishment of EMSA conditions provides an important framework to evaluate regulation of homeostatic or phase-specific genes that may influence virulence in Histoplasma and other dimorphic fungal pathogens.

Original languageEnglish
Pages (from-to)2238-2241
Number of pages4
JournalInfection and immunity
Volume70
Issue number4
DOIs
StatePublished - Apr 3 2002
Externally publishedYes

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