Gel shift assay of nuclear extracts from Histoplasma capsulatum demonstrates the presence of several DNA binding proteins

Atanas Ignatov; Elizabeth J. Keath

doi:10.1128/IAI.70.4.2238-2241.2002

Gel shift assay of nuclear extracts from Histoplasma capsulatum demonstrates the presence of several DNA binding proteins

Atanas Ignatov, Elizabeth J. Keath

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6 Scopus citations

Abstract

A gel shift assay was optimized to detect several general DNA binding proteins from Histoplasma capsulatum strain G217B. The electrophoretic mobility shift assay (EMSA) technique also detected protein(s) recognizing a pyrimidine-rich motif found in several Histoplasma promoters. Establishment of EMSA conditions provides an important framework to evaluate regulation of homeostatic or phase-specific genes that may influence virulence in Histoplasma and other dimorphic fungal pathogens.

Original language	English
Pages (from-to)	2238-2241
Number of pages	4
Journal	Infection and immunity
Volume	70
Issue number	4
DOIs	https://doi.org/10.1128/IAI.70.4.2238-2241.2002
State	Published - 2002

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10.1128/IAI.70.4.2238-2241.2002

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title = "Gel shift assay of nuclear extracts from Histoplasma capsulatum demonstrates the presence of several DNA binding proteins",

abstract = "A gel shift assay was optimized to detect several general DNA binding proteins from Histoplasma capsulatum strain G217B. The electrophoretic mobility shift assay (EMSA) technique also detected protein(s) recognizing a pyrimidine-rich motif found in several Histoplasma promoters. Establishment of EMSA conditions provides an important framework to evaluate regulation of homeostatic or phase-specific genes that may influence virulence in Histoplasma and other dimorphic fungal pathogens.",

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AU - Keath, Elizabeth J.

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AB - A gel shift assay was optimized to detect several general DNA binding proteins from Histoplasma capsulatum strain G217B. The electrophoretic mobility shift assay (EMSA) technique also detected protein(s) recognizing a pyrimidine-rich motif found in several Histoplasma promoters. Establishment of EMSA conditions provides an important framework to evaluate regulation of homeostatic or phase-specific genes that may influence virulence in Histoplasma and other dimorphic fungal pathogens.

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U2 - 10.1128/IAI.70.4.2238-2241.2002

DO - 10.1128/IAI.70.4.2238-2241.2002

M3 - Article

C2 - 11895995

AN - SCOPUS:0036127035

SN - 0019-9567

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EP - 2241

JO - Infection and immunity

JF - Infection and immunity

IS - 4

ER -

Gel shift assay of nuclear extracts from Histoplasma capsulatum demonstrates the presence of several DNA binding proteins

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