TY - JOUR
T1 - Gas phase studies of the interactions of Fe2+ with cysteine-containing peptides
AU - Nemirovskiy, O. V.
AU - Gross, M. L.
N1 - Funding Information:
This work was supported by the National Institutes of Health (Resource grant no. 2P41RR00954). The authors wish to thank Professor E. Unanue of the Department of Pathology at Washington University Medical School for sponsoring the peptide synthesis.
PY - 1998
Y1 - 1998
N2 - Gas-phase complexes of cysteine-containing peptides and Fe2+ were produced by fast atom bombardment and studied by tandem mass spectrometry. Specific and strong interactions of the iron and sulfur from the thiol group of the cysteine side chain are preserved in the gas phase and are the basis for highly specific fragmentation to give abundant [an - 2H + Fe]+ ions, where n is position of the cysteine residue from the N-terminus of peptide. Metal/peptide complexes containing more than one Cys residue were also investigated; they display similar chemistry upon collisionally activated decompositions, indicating that the Fe2+ ion primarily binds at cysteine sites.
AB - Gas-phase complexes of cysteine-containing peptides and Fe2+ were produced by fast atom bombardment and studied by tandem mass spectrometry. Specific and strong interactions of the iron and sulfur from the thiol group of the cysteine side chain are preserved in the gas phase and are the basis for highly specific fragmentation to give abundant [an - 2H + Fe]+ ions, where n is position of the cysteine residue from the N-terminus of peptide. Metal/peptide complexes containing more than one Cys residue were also investigated; they display similar chemistry upon collisionally activated decompositions, indicating that the Fe2+ ion primarily binds at cysteine sites.
UR - http://www.scopus.com/inward/record.url?scp=0032232657&partnerID=8YFLogxK
U2 - 10.1016/S1044-0305(98)00111-1
DO - 10.1016/S1044-0305(98)00111-1
M3 - Article
C2 - 9835073
AN - SCOPUS:0032232657
SN - 1044-0305
VL - 9
SP - 1285
EP - 1292
JO - Journal of the American Society for Mass Spectrometry
JF - Journal of the American Society for Mass Spectrometry
IS - 12
ER -