Gamma-aminobutyric acid (GABAA) receptor-function in a rat model of hepatic encephalopathy

Bennie L. Baker, A. Leslie Morrow, John Vergalla, Steven M. Paul, E. Anthony Jones

Research output: Contribution to journalArticlepeer-review

8 Scopus citations

Abstract

The functional activity of the gamma-aminobutyric acid (GABAA) receptor-chloride ionophore complex was studied in rats with hepatic encephalopathy (HE) secondary to thioacetamide-induced fulminant hepatic failure (FHF). Muscimol stimulation and benzodiazepine potentiation of GABA receptor-mediated36Cl- uptake into cerebral cortical synaptoneurosomes was compared in HE and control rats. [3H]Flumazenil binding assays were conducted to determine whether the levels of endogenous benzodiazepine-like ligands in extracts of cortex were increased with stages of encephalopathy in this animal model of HE. In both control and HE rats maximal uptake of36Cl- via the GABAA receptor complex occurred at muscimol concentrations of 30 μM. Potentiation of muscimol-stimulated36Cl- uptake into synaptoneurosomes by diazepam (5 μM) was equivalent in both groups. Aqueous extracts of proteolytically digested homogenates of cerebral cortices prepared from control and HE rats were effective in stimulating36Cl- uptake into synaptoneurosomes. Alkaline organic extracts of proteolytically digested homogenates of cerebral cortices from HE rats were more effective than corresponding extracts from controls at inhibiting the binding of [3H]flumazenil. Inhibition of [3H] fumazenil binding by organic extracts derived from the cerebral cortices of HE rats did not increase with progression of encephalopathy. The results show that muscimol-stimulated36Cl- uptake into synaptoneurosomes and, consequently, GABAA receptor-mediated chloride channel function are not significantly altered in the model of HE studied and are consistent with the hypothesis that HE results in an increased availability of one or more endogenous ligands which can augment GABA receptor-gated chloride conductance.

Original languageEnglish
Pages (from-to)185-193
Number of pages9
JournalMetabolic Brain Disease
Volume5
Issue number4
DOIs
StatePublished - Dec 1 1990

Keywords

  • benzodiazepine
  • chloride channel
  • gamma-aminobutyric acid
  • hepatic encephalopathy
  • synaptoneurosomes

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