TY - JOUR
T1 - Galectin-3, carotid plaque vulnerability, and potential effects of statin therapy
AU - Kadoglou, N. P.E.
AU - Sfyroeras, G. S.
AU - Spathis, A.
AU - Gkekas, C.
AU - Gastounioti, A.
AU - Mantas, G.
AU - Nikita, K. S.
AU - Karakitsos, P.
AU - Liapis, C. D.
N1 - Publisher Copyright:
© 2014 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.
PY - 2015/1/1
Y1 - 2015/1/1
N2 - Objectives Galectin-3, a member of galectines, a family of β-galactoside-specific lectins, has been reported to propagate vascular inflammation. The role of galectin-3 in carotid atherosclerosis is controversial. The aim of this study was to investigate the relationship of galectin-3 with plaque vulnerability in patients with high grade carotid stenosis. Methods This was a cross sectional study of patients undergoing carotid endarterectomy (CEA). Carotid plaques obtained from 78 consecutive patients (40 symptomatic [SG], 38 asymptomatic [AG]) undergoing CEA were histologically analyzed for galectin-3, macrophages (CD68) and laminin. Pre-operatively the biochemical profile and plaque echogenicity (gray-scale median, GSM) score were determined. Results There were no significant differences in clinical and demographic parameters between SG and AG (p >.05). The SG had a lower GSM score (44.21 ± 18.24 vs. 68.79 ± 28.79, p <.001) and a smaller positive stained area for galectin-3 (4.89 ± 1.60% vs. 12.01 ± 5.91%, p <.001) and laminin (0.88 ± 0.71% vs. 3.46 ± 2.12%, p <.001) than the AG. On the other hand, intra-plaque macrophage content was increased in SG (p <.001). For the whole cohort, symptomatic status was independently associated with intra-plaque contents of both galectin-3 (OR = 0.634, p <.001), and GSM score (OR = 0.750, p <.001). Notably, patients on long term statin treatment had elevated galectin-3 and lowered macrophage intra-plaque concentrations compared with those on short term treatment (p <.05). Conclusions A low galectin-3 intra-plaque concentration seems to correlate with clinically and ultrasonically defined unstable human carotid plaques. Long term statin treatment may induce increase of intra-plaque galectin-3 concentration mediating plaque stabilization.
AB - Objectives Galectin-3, a member of galectines, a family of β-galactoside-specific lectins, has been reported to propagate vascular inflammation. The role of galectin-3 in carotid atherosclerosis is controversial. The aim of this study was to investigate the relationship of galectin-3 with plaque vulnerability in patients with high grade carotid stenosis. Methods This was a cross sectional study of patients undergoing carotid endarterectomy (CEA). Carotid plaques obtained from 78 consecutive patients (40 symptomatic [SG], 38 asymptomatic [AG]) undergoing CEA were histologically analyzed for galectin-3, macrophages (CD68) and laminin. Pre-operatively the biochemical profile and plaque echogenicity (gray-scale median, GSM) score were determined. Results There were no significant differences in clinical and demographic parameters between SG and AG (p >.05). The SG had a lower GSM score (44.21 ± 18.24 vs. 68.79 ± 28.79, p <.001) and a smaller positive stained area for galectin-3 (4.89 ± 1.60% vs. 12.01 ± 5.91%, p <.001) and laminin (0.88 ± 0.71% vs. 3.46 ± 2.12%, p <.001) than the AG. On the other hand, intra-plaque macrophage content was increased in SG (p <.001). For the whole cohort, symptomatic status was independently associated with intra-plaque contents of both galectin-3 (OR = 0.634, p <.001), and GSM score (OR = 0.750, p <.001). Notably, patients on long term statin treatment had elevated galectin-3 and lowered macrophage intra-plaque concentrations compared with those on short term treatment (p <.05). Conclusions A low galectin-3 intra-plaque concentration seems to correlate with clinically and ultrasonically defined unstable human carotid plaques. Long term statin treatment may induce increase of intra-plaque galectin-3 concentration mediating plaque stabilization.
KW - Carotid atherosclerosis
KW - Galectin-3
KW - Plaque vulnerability
KW - Statins
KW - Stroke/transient ischemic attack
UR - http://www.scopus.com/inward/record.url?scp=84920570479&partnerID=8YFLogxK
U2 - 10.1016/j.ejvs.2014.10.009
DO - 10.1016/j.ejvs.2014.10.009
M3 - Article
C2 - 25457298
AN - SCOPUS:84920570479
SN - 1078-5884
VL - 49
SP - 4
EP - 9
JO - European Journal of Vascular and Endovascular Surgery
JF - European Journal of Vascular and Endovascular Surgery
IS - 1
ER -