TY - JOUR
T1 - Gi/o signaling and the palmitoyltransferase DHHC2 regulate palmitate cycling and shuttling of RGS7 family-binding protein
AU - Jia, Lixia
AU - Linder, Maurine E.
AU - Blumer, Kendall J.
PY - 2011/4/15
Y1 - 2011/4/15
N2 - R7BP (RGS7 family-binding protein) has been proposed to function in neurons as a palmitoylation-regulated protein that shuttles heterodimeric, G i/o α-specific GTPase-activating protein (GAP) complexes composed of Gβ5 and RGS7 (R7) isoforms between the plasma membrane and nucleus. To test this hypothesis we studied R7BP palmitoylation and localization in neuronal cells. We report that R7BP undergoes dynamic, signalregulated palmitate turnover; the palmitoyltransferase DHHC2 mediates de novo and turnover palmitoylation of R7BP; DHHC2 silencing redistributes R7BP from the plasma membrane to the nucleus; and Gi/o signaling inhibits R7BP depalmitoylation whereas Gi/o inactivation induces nuclear accumulation of R7BP. In concert with previous evidence, our findings suggest that agonist-induced changes in palmitoylation state facilitate GAP action by (i) promoting Giα depalmitoylation to create optimal GAP substrates, and (ii) inhibiting R7BP depalmitoylation to stabilize membrane association of R7-Gβ5 GAP complexes. Regulated palmitate turnover may also enable R7BP bound GAPs to shuttle between sites of low and high Gi/o activity or the plasma membrane and nucleus, potentially providing spatio-temporal control of signaling by Gi/o-coupled receptors.
AB - R7BP (RGS7 family-binding protein) has been proposed to function in neurons as a palmitoylation-regulated protein that shuttles heterodimeric, G i/o α-specific GTPase-activating protein (GAP) complexes composed of Gβ5 and RGS7 (R7) isoforms between the plasma membrane and nucleus. To test this hypothesis we studied R7BP palmitoylation and localization in neuronal cells. We report that R7BP undergoes dynamic, signalregulated palmitate turnover; the palmitoyltransferase DHHC2 mediates de novo and turnover palmitoylation of R7BP; DHHC2 silencing redistributes R7BP from the plasma membrane to the nucleus; and Gi/o signaling inhibits R7BP depalmitoylation whereas Gi/o inactivation induces nuclear accumulation of R7BP. In concert with previous evidence, our findings suggest that agonist-induced changes in palmitoylation state facilitate GAP action by (i) promoting Giα depalmitoylation to create optimal GAP substrates, and (ii) inhibiting R7BP depalmitoylation to stabilize membrane association of R7-Gβ5 GAP complexes. Regulated palmitate turnover may also enable R7BP bound GAPs to shuttle between sites of low and high Gi/o activity or the plasma membrane and nucleus, potentially providing spatio-temporal control of signaling by Gi/o-coupled receptors.
UR - http://www.scopus.com/inward/record.url?scp=79953871598&partnerID=8YFLogxK
U2 - 10.1074/jbc.M110.193763
DO - 10.1074/jbc.M110.193763
M3 - Article
C2 - 21343290
AN - SCOPUS:79953871598
VL - 286
SP - 13695
EP - 13703
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
SN - 0021-9258
IS - 15
ER -