Gi/o signaling and the palmitoyltransferase DHHC2 regulate palmitate cycling and shuttling of RGS7 family-binding protein

Lixia Jia, Maurine E. Linder, Kendall J. Blumer

Research output: Contribution to journalArticlepeer-review

20 Scopus citations

Abstract

R7BP (RGS7 family-binding protein) has been proposed to function in neurons as a palmitoylation-regulated protein that shuttles heterodimeric, G i/o α-specific GTPase-activating protein (GAP) complexes composed of Gβ5 and RGS7 (R7) isoforms between the plasma membrane and nucleus. To test this hypothesis we studied R7BP palmitoylation and localization in neuronal cells. We report that R7BP undergoes dynamic, signalregulated palmitate turnover; the palmitoyltransferase DHHC2 mediates de novo and turnover palmitoylation of R7BP; DHHC2 silencing redistributes R7BP from the plasma membrane to the nucleus; and Gi/o signaling inhibits R7BP depalmitoylation whereas Gi/o inactivation induces nuclear accumulation of R7BP. In concert with previous evidence, our findings suggest that agonist-induced changes in palmitoylation state facilitate GAP action by (i) promoting Giα depalmitoylation to create optimal GAP substrates, and (ii) inhibiting R7BP depalmitoylation to stabilize membrane association of R7-Gβ5 GAP complexes. Regulated palmitate turnover may also enable R7BP bound GAPs to shuttle between sites of low and high Gi/o activity or the plasma membrane and nucleus, potentially providing spatio-temporal control of signaling by Gi/o-coupled receptors.

Original languageEnglish
Pages (from-to)13695-13703
Number of pages9
JournalJournal of Biological Chemistry
Volume286
Issue number15
DOIs
StatePublished - Apr 15 2011

Fingerprint Dive into the research topics of 'G<sub>i/o</sub> signaling and the palmitoyltransferase DHHC2 regulate palmitate cycling and shuttling of RGS7 family-binding protein'. Together they form a unique fingerprint.

Cite this