TY - JOUR
T1 - G-quadruplex formation of thrombin-binding aptamer detected by electrospray ionization mass spectrometry
AU - Vairamani, M.
AU - Gross, Michael L.
PY - 2003/1/8
Y1 - 2003/1/8
N2 - Electrospray ionization mass spectrometry detected the formation of the G-quadruplex structure of the thrombin-binding aptamer, d(GGTTGGTGTGGTTGG), and established its specific interaction with metal ions. One piece of evidence that the bonding in the gas phase is via the G-quadruplex form is the enhanced binding, with respect to other metal ions, of the aptamer with Sr2+, Pb2+, Ba2+, and K+, which are of similar size. Another is the lack of specific binding with controls in which the G's are replaced with A's. The most convincing evidence is the extent of H/D exchange of the gas-phase aptamer as compared to that bound to K+ and Sr2+. The latter two complexes exchange six and nine fewer H's, indicating a significant increase in protection upon binding to the metals. Mass spectrometry will be an important tool in understanding G-quadruplexes, which are particularly important in DNA telomers.
AB - Electrospray ionization mass spectrometry detected the formation of the G-quadruplex structure of the thrombin-binding aptamer, d(GGTTGGTGTGGTTGG), and established its specific interaction with metal ions. One piece of evidence that the bonding in the gas phase is via the G-quadruplex form is the enhanced binding, with respect to other metal ions, of the aptamer with Sr2+, Pb2+, Ba2+, and K+, which are of similar size. Another is the lack of specific binding with controls in which the G's are replaced with A's. The most convincing evidence is the extent of H/D exchange of the gas-phase aptamer as compared to that bound to K+ and Sr2+. The latter two complexes exchange six and nine fewer H's, indicating a significant increase in protection upon binding to the metals. Mass spectrometry will be an important tool in understanding G-quadruplexes, which are particularly important in DNA telomers.
UR - http://www.scopus.com/inward/record.url?scp=0037425570&partnerID=8YFLogxK
U2 - 10.1021/ja0284299
DO - 10.1021/ja0284299
M3 - Article
C2 - 12515502
AN - SCOPUS:0037425570
SN - 0002-7863
VL - 125
SP - 42
EP - 43
JO - Journal of the American Chemical Society
JF - Journal of the American Chemical Society
IS - 1
ER -