FYCO1 regulates accumulation of post-mitotic midbodies by mediating LC3-dependent midbody degradation

Lai Kuan Dionne, Eric Peterman, John Schiel, Paulius Gibieža, Vytenis Arvydas Skeberdis, Antonio Jimeno, Xiao Jing Wang, Rytis Prekeris

Research output: Contribution to journalArticlepeer-review

9 Scopus citations

Abstract

The post-mitotic midbody (MB) is a remnant of cytokinesis that can be asymmetrically inherited by one of the daughter cells following cytokinesis. Until recently, the MB was thought to be degraded immediately following cytokinesis. However, recent evidence suggests that the MB is a protein-rich organelle that accumulates in stem cell and cancer cell populations, indicating that itmay have postmitotic functions. Here, we investigate the role of FYCO1, an LC3- binding protein (herein, LC3 refers to MAP1LC3B), and its function in regulating the degradation of post-mitotic MBs. We show that FYCO1 is responsible for formation of LC3-containing membrane around the post-mitotic MB and that FYCO1 knockdown increases MB accumulation. Although MBs accumulate in the stem-cell-like population of squamous cell carcinomas, FYCO1 depletion does not affect the clonogenicity of these cells. Instead, MB accumulation leads to an increase in anchorage-independent growth and invadopodia formation in HeLa cells and squamous carcinoma cells. Collectively, our data suggest that FYCO1 regulates MB degradation, and we present the first evidence that cancer invasiveness is a feature that can be modulated by the accumulation of MBs in cancer stem cells.

Original languageEnglish
Pages (from-to)4051-4062
Number of pages12
JournalJournal of cell science
Volume130
Issue number23
DOIs
StatePublished - Jan 1 2017
Externally publishedYes

Keywords

  • Autophagy
  • Cytokinesis
  • Midbody
  • Post-mitotic accumulation
  • Post-mitotic degradation

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