TY - GEN
T1 - Fundamental limits of measuring single-molecule rotational mobility
AU - Zhang, Oumeng
AU - Lew, Matthew D.
N1 - Publisher Copyright:
© COPYRIGHT SPIE. Downloading of the abstract is permitted for personal use only.
PY - 2019
Y1 - 2019
N2 - Various methods exist for measuring molecular orientation, thereby providing insight into biochemical activities at nanoscale. Since fluorescence intensity and not electric field is detected, these methods are limited to measuring even-order moments of molecular orientation. However, any measurement noise, for example photon shot noise, will result in nonzero measurements of any of these even-order moments, thereby causing rotationally-free molecules to appear to be partially constrained. Here, we build a model to quantify measurement errors in rotational mobility. Our theoretical framework enables scientists to choose the optimal single-molecule orientation measurement technique for any desired measurement accuracy and photon budget.
AB - Various methods exist for measuring molecular orientation, thereby providing insight into biochemical activities at nanoscale. Since fluorescence intensity and not electric field is detected, these methods are limited to measuring even-order moments of molecular orientation. However, any measurement noise, for example photon shot noise, will result in nonzero measurements of any of these even-order moments, thereby causing rotationally-free molecules to appear to be partially constrained. Here, we build a model to quantify measurement errors in rotational mobility. Our theoretical framework enables scientists to choose the optimal single-molecule orientation measurement technique for any desired measurement accuracy and photon budget.
KW - Measurement bias
KW - Orientational dynamics
KW - Photon shot noise
KW - Single-molecule fluorescence imaging
UR - http://www.scopus.com/inward/record.url?scp=85066761707&partnerID=8YFLogxK
U2 - 10.1117/12.2506712
DO - 10.1117/12.2506712
M3 - Conference contribution
AN - SCOPUS:85066761707
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Single Molecule Spectroscopy and Superresolution Imaging XII
A2 - Gryczynski, Zygmunt K.
A2 - Gregor, Ingo
A2 - Koberling, Felix
PB - SPIE
T2 - Single Molecule Spectroscopy and Superresolution Imaging XII 2019
Y2 - 2 February 2019 through 3 February 2019
ER -