Fundamental limits of measuring single-molecule rotational mobility

Oumeng Zhang, Matthew D. Lew

Research output: Chapter in Book/Report/Conference proceedingConference contributionpeer-review

Abstract

Various methods exist for measuring molecular orientation, thereby providing insight into biochemical activities at nanoscale. Since fluorescence intensity and not electric field is detected, these methods are limited to measuring even-order moments of molecular orientation. However, any measurement noise, for example photon shot noise, will result in nonzero measurements of any of these even-order moments, thereby causing rotationally-free molecules to appear to be partially constrained. Here, we build a model to quantify measurement errors in rotational mobility. Our theoretical framework enables scientists to choose the optimal single-molecule orientation measurement technique for any desired measurement accuracy and photon budget.

Original languageEnglish
Title of host publicationSingle Molecule Spectroscopy and Superresolution Imaging XII
EditorsZygmunt K. Gryczynski, Ingo Gregor, Felix Koberling
PublisherSPIE
ISBN (Electronic)9781510624108
DOIs
StatePublished - 2019
EventSingle Molecule Spectroscopy and Superresolution Imaging XII 2019 - San Francisco, United States
Duration: Feb 2 2019Feb 3 2019

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
Volume10884
ISSN (Print)1605-7422

Conference

ConferenceSingle Molecule Spectroscopy and Superresolution Imaging XII 2019
Country/TerritoryUnited States
CitySan Francisco
Period02/2/1902/3/19

Keywords

  • Measurement bias
  • Orientational dynamics
  • Photon shot noise
  • Single-molecule fluorescence imaging

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