Functional size of the human asialoglycoprotein receptor as determined by radiation inactivation

A. L. Schwartz, C. J. Steer, E. S. Kempner

Research output: Contribution to journalArticle

10 Scopus citations

Abstract

The radiation target size of the functional asialoglycoprotein receptor (ASGP-R) in purified plasma membranes was determined for human liver and the human hepatoma cell line, Hep G2, by evaluation of the binding of 125I-asialoorosomucoid. Identical inactivation curves were observed for ASGP-R from normal human liver and post-mortem human liver and exhibited a functional unit of 70,000 daltons. The human hepatoma Hep G2 ASGP-R, in contrast, showed a target size of 140,000 daltons. These results suggest that the functional ligand-binding unit of the human ASGP-R is a multimer of the ~ 34,000-dalton unglycosylated receptor polypeptide (Schwartz, A.L, and Rup, D. (1983) J. Biol. Chem. 258, 11249-11255).

Original languageEnglish
Pages (from-to)12025-12029
Number of pages5
JournalJournal of Biological Chemistry
Volume259
Issue number19
StatePublished - Jan 1 1984
Externally publishedYes

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