TY - JOUR
T1 - Functional PDF Signaling in the Drosophila Circadian Neural Circuit Is Gated by Ral A-Dependent Modulation
AU - Klose, Markus
AU - Duvall, Laura B.
AU - Li, Weihua
AU - Liang, Xitong
AU - Ren, Chi
AU - Steinbach, Joe Henry
AU - Taghert, Paul H.
N1 - Funding Information:
We thank Tom Schwartz, Michael Nitabach, the Bloomington Stock Center, and the VDRC for Drosophila stocks; Steve Ferguson for plasmids; Katherine Abruzzi and Michael Rosbash for sharing unpublished information; and Dongkook Park, Erik Herzog, and Stephan Dissel for critically reading a draft of this manuscript. We thank Jennifer Trigg for excellent technical assistance, Dennis Oakley of the Bakewell Neuroimaging Laboratory and the Washington University Center for Cellular Imaging (WUCCI) for assistance, and members of the laboratory for advice and helpful comments. L.B.D. was supported by NIH 5-T32-GM08151-27 and 5-T32-EY013360-10. The work was supported by a grant from the NIH (R01 MH067122) to P.H.T.
Publisher Copyright:
© 2016 Elsevier Inc.
PY - 2016/5/18
Y1 - 2016/5/18
N2 - The neuropeptide PDF promotes the normal sequencing of circadian behavioral rhythms in Drosophila, but its signaling mechanisms are not well understood. We report daily rhythmicity in responsiveness to PDF in critical pacemakers called small LNvs. There is a daily change in potency, as great as 10-fold higher, around dawn. The rhythm persists in constant darkness and does not require endogenous ligand (PDF) signaling or rhythmic receptor gene transcription. Furthermore, rhythmic responsiveness reflects the properties of the pacemaker cell type, not the receptor. Dopamine responsiveness also cycles, in phase with that of PDF, in the same pacemakers, but does not cycle in large LNv. The activity of RalA GTPase in s-LNv regulates PDF responsiveness and behavioral locomotor rhythms. Additionally, cell-autonomous PDF signaling reversed the circadian behavioral effects of lowered RalA activity. Thus, RalA activity confers high PDF responsiveness, providing a daily gate around the dawn hours to promote functional PDF signaling. Klose et al. demonstrate daily rhythmic sensitivity to neuropeptide PDF in the Drosophila circadian neural circuit: this rhythm gates PDF modulation of behavior to the period around dawn. The rhythm is cell type specific, ligand independent, and regulated by the RalA GTPase.
AB - The neuropeptide PDF promotes the normal sequencing of circadian behavioral rhythms in Drosophila, but its signaling mechanisms are not well understood. We report daily rhythmicity in responsiveness to PDF in critical pacemakers called small LNvs. There is a daily change in potency, as great as 10-fold higher, around dawn. The rhythm persists in constant darkness and does not require endogenous ligand (PDF) signaling or rhythmic receptor gene transcription. Furthermore, rhythmic responsiveness reflects the properties of the pacemaker cell type, not the receptor. Dopamine responsiveness also cycles, in phase with that of PDF, in the same pacemakers, but does not cycle in large LNv. The activity of RalA GTPase in s-LNv regulates PDF responsiveness and behavioral locomotor rhythms. Additionally, cell-autonomous PDF signaling reversed the circadian behavioral effects of lowered RalA activity. Thus, RalA activity confers high PDF responsiveness, providing a daily gate around the dawn hours to promote functional PDF signaling. Klose et al. demonstrate daily rhythmic sensitivity to neuropeptide PDF in the Drosophila circadian neural circuit: this rhythm gates PDF modulation of behavior to the period around dawn. The rhythm is cell type specific, ligand independent, and regulated by the RalA GTPase.
KW - Circadian rhythms
KW - Dopamine
KW - Drosophila
KW - GPCR
KW - PDF
KW - RalA
UR - http://www.scopus.com/inward/record.url?scp=84964977887&partnerID=8YFLogxK
U2 - 10.1016/j.neuron.2016.04.002
DO - 10.1016/j.neuron.2016.04.002
M3 - Article
C2 - 27161526
AN - SCOPUS:84964977887
SN - 0896-6273
VL - 90
SP - 781
EP - 794
JO - Neuron
JF - Neuron
IS - 4
ER -