TY - CHAP
T1 - Functional Metagenomics to Study Antibiotic Resistance
AU - Mahmud, Bejan
AU - Boolchandani, Manish
AU - Patel, Sanket
AU - Dantas, Gautam
N1 - Publisher Copyright:
© 2023, The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
PY - 2023
Y1 - 2023
N2 - The construction and screening of metagenomic expression libraries have a great potential to identify novel genes with desired functions. Here, we describe metagenomic library preparation from fecal DNA, screening of libraries for antibiotic resistance genes (ARGs), massively parallel DNA sequencing of the enriched DNA fragments, and a computational pipeline for high-throughput assembly and annotation of functionally selected DNA.
AB - The construction and screening of metagenomic expression libraries have a great potential to identify novel genes with desired functions. Here, we describe metagenomic library preparation from fecal DNA, screening of libraries for antibiotic resistance genes (ARGs), massively parallel DNA sequencing of the enriched DNA fragments, and a computational pipeline for high-throughput assembly and annotation of functionally selected DNA.
KW - Antibiotic resistance genes
KW - Functional metagenomics
KW - Functional selections
KW - High-throughput assembly
KW - Massively parallel DNA sequencing
KW - PARFuMS
KW - Profile HMM-based annotation
KW - Resfams
KW - Resistome
UR - http://www.scopus.com/inward/record.url?scp=85143088235&partnerID=8YFLogxK
U2 - 10.1007/978-1-0716-2855-3_21
DO - 10.1007/978-1-0716-2855-3_21
M3 - Chapter
C2 - 36445596
AN - SCOPUS:85143088235
T3 - Methods in Molecular Biology
SP - 379
EP - 401
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -